在 Göttingen 小型猪中检测一种用于干细胞和治疗的新型临床脑内微量注射仪器的安全性和功能。
Safety and function of a new clinical intracerebral microinjection instrument for stem cells and therapeutics examined in the Göttingen minipig.
作者信息
Bjarkam Carsten R, Glud Andreas N, Margolin Lee, Reinhart Karl, Franklin Ron, Deding Dorthe, Ettrup Kåre S, Fitting Lise M, Nielsen Mette S, Sørensen Jens-Christian H, Cunningham Miles G
机构信息
Department of Neurobiology, Institute of Anatomy, University of Aarhus, Aarhus, Denmark.
出版信息
Stereotact Funct Neurosurg. 2010;88(1):56-63. doi: 10.1159/000268743. Epub 2009 Dec 24.
BACKGROUND
A new intracerebral microinjection instrument (IMI) allowing multiple electrophysiologically guided microvolume injections from a single proximal injection path in rats has been adapted to clinical use by coupling the IMI to an FHC microTargeting Manual Drive, designed to be used with standard stereotactic frame-based systems and FHC frameless microTargeting Platforms.
METHODS
The function and safety of the device was tested by conducting bilateral electrophysiologically guided microinjections of fluorescent microspheres in the substantia nigra of 4 Göttingen minipigs.
RESULTS
The device was easy to handle and enabled accurate electrophysiologically guided targeting of the substantia nigra with minimal local tissue damage.
CONCLUSION
The IMI is suitable for clinical use and may prove useful for various stereotactic procedures that require high levels of precision and/or three-dimensional distribution of therapeutics within the brain.
背景
一种新型脑内微注射仪器（IMI）可在大鼠中通过单一近端注射路径进行多次电生理引导的微量注射，通过将IMI与FHC微靶向手动驱动器耦合，已将其应用于临床，该驱动器设计用于基于标准立体定向框架的系统和FHC无框架微靶向平台。
方法
通过在4只哥廷根小型猪的黑质中进行双侧电生理引导的荧光微球微量注射，测试该装置的功能和安全性。
结果
该装置易于操作，能够在对局部组织损伤最小的情况下，通过电生理引导准确靶向黑质。
结论
IMI适用于临床应用，对于各种需要高精度和/或脑内治疗药物三维分布的立体定向手术可能有用。
Zotero 插件