Yaseen Mohammad A, Srinivasan Vivek J, Sakadzić Sava, Wu Weicheng, Ruvinskaya Svetlana, Vinogradov Sergei A, Boas David A
Photon Migration Imaging Laboratory, MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Charlestown, MA 02129, USA
Opt Express. 2009 Dec 7;17(25):22341-50. doi: 10.1364/OE.17.022341.
Evaluating cerebral oxygenation is of critical importance for the understanding of brain function and several neuropathologies. Although several techniques exist for measuring cerebral oxygenation in vivo, the most widely accepted techniques offer limited spatial resolution. We have developed a confocal imaging system for minimally invasive measurement of oxygen tension (pO(2)) in cerebral microvessels with high spatial and temporal resolution. The system relies on the phosphorescence quenching method using exogenous porphyrin-based dendritic oxygen probes. Here we present high-resolution phosphorescence images of cortical microvasculature and temporal pO(2) profiles from multiple locations in response to varied fraction of inspired oxygen and functional activation.
评估脑氧合对于理解脑功能和多种神经病理学至关重要。尽管存在多种体内测量脑氧合的技术，但最广泛接受的技术空间分辨率有限。我们开发了一种共聚焦成像系统，用于以高空间和时间分辨率微创测量脑微血管中的氧张力（pO₂）。该系统依赖于使用基于外源性卟啉的树枝状氧探针的磷光猝灭方法。在此，我们展示了皮质微血管的高分辨率磷光图像以及多个位置响应不同吸入氧分数和功能激活的时间pO₂ 曲线。
