Shonat R D, Wilson D F, Riva C E, Pawlowski M
Appl Opt. 1992 Jul 1;31(19):3711-8. doi: 10.1364/AO.31.003711.
The oxygen tension in the vessels of the retina and optic nerve head has been measured noninvasively with a new phosphorescence imaging method. A phosphorescent oxygen-dependent probe, injected into the bloodstream of cats, was excited with a flash of light and the phosphorescence lifetime of the probe was measured. A simple Stern-Volmer relationship was used to convert lifetime to oxygen tension, and two-dimensional maps of intravascular oxygen tension were produced. We describe the equipment and the methodology for obtaining oxygen maps.
利用一种新的磷光成像方法，已对视网膜血管和视神经乳头中的氧分压进行了无创测量。将一种磷光氧依赖性探针注入猫的血液中，用闪光激发该探针，并测量其磷光寿命。利用简单的斯特恩-沃尔默关系将寿命转换为氧分压，生成了血管内氧分压的二维图。我们描述了获取氧图的设备和方法。
