MC3T3成骨细胞的磁悬浮作为微重力的地面模拟
Magnetic Levitation of MC3T3 Osteoblast Cells as a Ground-Based Simulation of Microgravity.
作者信息
Hammer Bruce E, Kidder Louis S, Williams Philip C, Xu Wayne Wenzhong
机构信息
Department of Radiology, University of Minnesota, 420 Delaware St., MMC 292, Minneapolis, MN 55455, USA, URL: www.ciamr.umn.edu.
出版信息
Microgravity Sci Technol. 2009 Nov;21(4):311-318. doi: 10.1007/s12217-008-9092-6.
Abstract
Diamagnetic samples placed in a strong magnetic field and a magnetic field gradient experience a magnetic force. Stable magnetic levitation occurs when the magnetic force exactly counter balances the gravitational force. Under this condition, a diamagnetic sample is in a simulated microgravity environment. The purpose of this study is to explore if MC3T3-E1 osteoblastic cells can be grown in magnetically simulated hypo-g and hyper-g environments and determine if gene expression is differentially expressed under these conditions. The murine calvarial osteoblastic cell line, MC3T3-E1, grown on Cytodex-3 beads, were subjected to a net gravitational force of 0, 1 and 2 g in a 17 T superconducting magnet for 2 days. Microarray analysis of these cells indicated that gravitational stress leads to up and down regulation of hundreds of genes. The methodology of sustaining long-term magnetic levitation of biological systems are discussed.
摘要
置于强磁场和磁场梯度中的抗磁性样品会受到磁力作用。当磁力恰好与重力平衡时，就会出现稳定的磁悬浮现象。在此条件下，抗磁性样品处于模拟微重力环境中。本研究的目的是探索MC3T3-E1成骨细胞能否在磁模拟低重力和高重力环境中生长，并确定在这些条件下基因表达是否存在差异。将生长在Cytodex-3微载体珠上的小鼠颅盖成骨细胞系MC3T3-E1置于17 T超导磁体中，分别承受0、1和2 g的净重力，持续2天。对这些细胞进行微阵列分析表明，重力应激会导致数百个基因的上调和下调。文中还讨论了维持生物系统长期磁悬浮的方法。
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