Hammer Bruce E, Kidder Louis S, Williams Philip C, Xu Wayne Wenzhong
Department of Radiology, University of Minnesota, 420 Delaware St., MMC 292, Minneapolis, MN 55455, USA, URL: www.ciamr.umn.edu.
Microgravity Sci Technol. 2009 Nov;21(4):311-318. doi: 10.1007/s12217-008-9092-6.
Diamagnetic samples placed in a strong magnetic field and a magnetic field gradient experience a magnetic force. Stable magnetic levitation occurs when the magnetic force exactly counter balances the gravitational force. Under this condition, a diamagnetic sample is in a simulated microgravity environment. The purpose of this study is to explore if MC3T3-E1 osteoblastic cells can be grown in magnetically simulated hypo-g and hyper-g environments and determine if gene expression is differentially expressed under these conditions. The murine calvarial osteoblastic cell line, MC3T3-E1, grown on Cytodex-3 beads, were subjected to a net gravitational force of 0, 1 and 2 g in a 17 T superconducting magnet for 2 days. Microarray analysis of these cells indicated that gravitational stress leads to up and down regulation of hundreds of genes. The methodology of sustaining long-term magnetic levitation of biological systems are discussed.
置于强磁场和磁场梯度中的抗磁性样品会受到磁力作用。当磁力恰好与重力平衡时，就会出现稳定的磁悬浮现象。在此条件下，抗磁性样品处于模拟微重力环境中。本研究的目的是探索MC3T3-E1成骨细胞能否在磁模拟低重力和高重力环境中生长，并确定在这些条件下基因表达是否存在差异。将生长在Cytodex-3微载体珠上的小鼠颅盖成骨细胞系MC3T3-E1置于17 T超导磁体中，分别承受0、1和2 g的净重力，持续2天。对这些细胞进行微阵列分析表明，重力应激会导致数百个基因的上调和下调。文中还讨论了维持生物系统长期磁悬浮的方法。
