Zhang Bin, Li Xianglin, Zhou Xiaojie, Lou ChenGe, Wang Shenghang, Lv Huanhuan, Zhang Gejing, Fang Yanwen, Yin Dachuan, Shang Peng
Research & Development Institute of Northwestern Polytechnical University in Shenzhen, Shenzhen 518057, China.
School of Life Science, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.
iScience. 2023 Jul 13;26(8):107365. doi: 10.1016/j.isci.2023.107365. eCollection 2023 Aug 18.
Osteocytes are the mechano-sensors of bones. Large gradient high-static magnetic fields (LG-HMFs) produce stable, high-precision, and non-attenuation mechanical forces. We discovered that magnetic forces opposite to gravity inhibited MLO-Y4 osteocyte proliferation and viability by inducing structural damage and apoptosis. In contrast, magnetic force loading in the same direction as that of gravity promoted the proliferation and inhibited apoptosis of MLO-Y4 osteocytes. Differentially expressed gene (DEG) analysis after magnetic force stimulation indicated that the ECM-integrin-CSK axis responded most significantly to mechanical signals. was the most significant DEG between the 12 T upward and downward groups, showing the highest correlation with the Wnt pathway according to the STRING protein interaction database. Explaining the cellular and molecular mechanisms by which mechanical stimuli influence bone remodeling is currently the focus of osteocyte-related research. Our findings provide insights into the effects of LG-HMFs on bone cells, which have further implications in clinical practice.
骨细胞是骨骼的机械传感器。大梯度高静磁场（LG-HMFs）能产生稳定、高精度且无衰减的机械力。我们发现，与重力方向相反的磁力通过诱导结构损伤和细胞凋亡抑制了MLO-Y4骨细胞的增殖和活力。相反，与重力方向相同的磁力加载促进了MLO-Y4骨细胞的增殖并抑制了其凋亡。磁力刺激后的差异表达基因（DEG）分析表明，细胞外基质-整合素-CSK轴对机械信号的反应最为显著。 是12T向上和向下组之间最显著的差异表达基因，根据STRING蛋白质相互作用数据库，其与Wnt通路的相关性最高。解释机械刺激影响骨重塑的细胞和分子机制是目前骨细胞相关研究的重点。我们的研究结果为LG-HMFs对骨细胞的影响提供了见解，这在临床实践中具有进一步的意义。
