First Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Munich, 80337, Germany.
Arch Gynecol Obstet. 2011 Feb;283(2):223-30. doi: 10.1007/s00404-009-1340-5. Epub 2010 Jan 6.
Inhibins and activins are important regulators of the female reproductive system. Recently, a novel inhibin subunit, named betaE, has been identified and shown to be expressed in several human tissues. However, only limited data on the expression of this novel inhibin-betaE subunit in normal and pathological human placenta as well as and human chorionic carcinoma cell lines exist.
Tissue specimens of normal, preeclamptic and HELLP pregnancies (n = 18) were obtained at the course of an cesarean section. Normal and pathological placental tissues as well as chorionic carcinoma cells (BeWo and JEG) were analyzed by using immunohistochemistry and RT-PCR.
Expression of the inhibin betaE subunit could be demonstrated at the protein level by means of immunohistochemical evaluation and at the transcriptional level by betaE-specific RT-PCR analysis. The immunoreactive score for inhibin-betaE did not show any significant differences between normal, preeclamptic and HELLP tissue in extravillous trophoblast and syncytiotrophoblast cells. Expression of inhibin betaE could further be demonstrated for the human chorionic carcinoma cell lines JEG and BeWo.
We demonstrated that inhibin-betaE is expressed in normal and pathological human placenta tissues. Although the precise role of this novel inhibin subunit for human placenta development is quite unclear, similarities with the well-characterized betaA- and betaB-subunits suggest an involvement in autocrine/paracrine signaling pathways, angiogenesis, decidualization and tissue remodeling under normal as well as malignant conditions. Additionally, the human chorionic carcinoma cell lines JEG and BeWo synthesize this subunit and therefore can be used as a cell culture model for further functional analysis of this subunit in human placental tissue.
抑制素和激活素是女性生殖系统的重要调节因子。最近，一种新的抑制素亚基，命名为βE，已被鉴定并显示在几种人体组织中表达。然而，关于这种新的抑制素-βE 亚基在正常和病理性人胎盘以及人绒毛膜癌细胞系中的表达，仅有有限的数据。
在剖宫产过程中获得了正常、子痫前期和 HELLP 妊娠（n=18）的组织标本。通过免疫组织化学和 RT-PCR 分析正常和病理性胎盘组织以及绒毛膜癌细胞（BeWo 和 JEG）。
通过免疫组织化学评估和βE 特异性 RT-PCR 分析，在蛋白质水平和转录水平均能证明抑制素βE 亚基的表达。在绒毛外滋养层和合体滋养层细胞中，抑制素-βE 的免疫反应评分在正常、子痫前期和 HELLP 组织之间没有显示出任何显著差异。抑制素βE 的表达也可以在人绒毛膜癌细胞系 JEG 和 BeWo 中得到证明。
我们证明了抑制素-βE 在正常和病理性人胎盘组织中表达。虽然这个新的抑制素亚基对人胎盘发育的确切作用尚不清楚，但与已被充分描述的βA-和βB-亚基的相似性表明，它参与了自分泌/旁分泌信号通路、血管生成、蜕膜化和组织重塑，无论是在正常还是恶性条件下。此外，人绒毛膜癌细胞系 JEG 和 BeWo 合成这种亚基，因此可以用作细胞培养模型，以进一步分析这种亚基在人胎盘组织中的功能。
