School of Public Health, Shandong University, Jinan, China.
Environ Toxicol. 2011 Jun;26(3):307-14. doi: 10.1002/tox.20560.
Previous studies have shown that tributyltin could act as an endocrine disruptor in mammals. However, the data on the low-dose effect of tributyltin in animals are still lacking. The objective of this study was to demonstrate the endocrine disruption induced by low levels of tributyltin chloride (TBTCl) in male KM mice. The animals were treated with 0.05 or 0.5 mg TBTCl/kg body weight/3 days from postnatal days (PNDs) 24 to 45, and killed on PNDs 49 and 84, respectively. Mice treated with 0.5 mg TBTCl/kg exhibited decreased serum and intratesticular testosterone (T) levels on PND 49 and then followed by an obvious recovery on PND 84. Furthermore, mice treated with 0.05 mg TBTCl/kg showed reduced serum 17β-estradiol (E2) levels on PND 49. However, treatments with TBTCl resulted in a dose-dependent increase in serum E2 concentration of the mice on PND 84. Administration of TBTCl also decreased levels of serum luteinizing hormone and intratesticular E2 on PND 84. In addition, mice exposed to 0.05 mg/kg TBTCl exhibited an increase in body weight in the late stage of the experiment. These results indicate that treatment with low doses of TBTCl could disturb hormone homeostasis and body weight gain in rodents, and exposure to different levels of TBTCl might have different effects on changing some physiologic parameters.
先前的研究表明三丁基锡（Tributyltin，TBT）可能在哺乳动物中充当内分泌干扰物。然而，关于 TBT 在动物中低剂量效应的数据仍然缺乏。本研究旨在证明三丁基氯化锡（Tributyltin chloride，TBTCl）在雄性 KM 小鼠中的低水平暴露会引发内分泌干扰。动物在出生后第 24 至 45 天期间，每天经口给予 0.05 或 0.5 mg TBTCl/kg 体重，分别于第 49 天和第 84 天处死。经 0.5 mg TBTCl/kg 处理的小鼠在第 49 天表现出血清和睾丸内睾酮（Testosterone，T）水平降低，随后在第 84 天明显恢复。此外，经 0.05 mg TBTCl/kg 处理的小鼠在第 49 天表现出血清 17β-雌二醇（17β-estradiol，E2）水平降低。然而，TBTCl 处理导致第 84 天血清 E2 浓度呈剂量依赖性增加。TBTCl 的给药还降低了第 84 天血清促黄体生成素和睾丸内 E2 的水平。此外，暴露于 0.05 mg/kg TBTCl 的小鼠在实验后期体重增加。这些结果表明，低剂量 TBTCl 的处理可能会干扰啮齿动物的激素平衡和体重增加，并且暴露于不同水平的 TBTCl 可能会对改变某些生理参数产生不同的影响。
