Si Jiliang, Li Peng, Xin Quanbing, Li Xuewen, An Lihong, Li Jie
Department of Environmental Health, School of Public Health, Shandong University, Jinan, China.
Environ Toxicol. 2015 Jan;30(1):44-52. doi: 10.1002/tox.21892. Epub 2013 Aug 2.
Exposure to endocrine disruptors (EDs) during early development might lead to adverse health outcomes later in life. Tributyltin (TBT), a proven ED, is widely used in consumer goods and industrial products. Herein we demonstrate the effects of low doses of tributyltin chloride (TBTCl) on reproduction of male KM mice. Pregnant mice were administered by gavage with 0, 1, 10, or 100 μg TBTCl/kg body weight/day from day 6 of pregnancy through the period of lactation. TBTCl dramatically decreased sperm counts and motility on postnatal days (PNDs) 49 and 152. Meanwhile, a significant increase in sperm abnormality was observed in exposed mice on PND 49, but comparable to that in the control on PND 152. The histopathological analysis of testes of treated animals showed a dose-dependent increase in sloughing of germ cells in seminiferous tubules. Mice treated with 10 μg TBTCl/kg exhibited decreased intratesticular 17β-estradiol (E2) levels on PND 49, and then followed by an obvious recovery on PND 152. While, no significant differences in serum E2, testosterone (T) levels and intratesticular T levels were detectable between control and TBTCl-exposed offspring at the sacrifice. These results suggest that perinatal TBTCl exposure is implicated in causing long lasting alterations in male reproductive system and these changes may persist far into adulthood.
在早期发育过程中接触内分泌干扰物（EDs）可能会导致日后出现不良健康后果。三丁基锡（TBT）是一种已被证实的内分泌干扰物，广泛应用于消费品和工业产品中。在此，我们展示了低剂量氯化三丁基锡（TBTCl）对雄性KM小鼠生殖的影响。从怀孕第6天到哺乳期，对怀孕小鼠经口灌胃给予0、1、10或100μg TBTCl/千克体重/天。TBTCl显著降低了出生后第49天和152天的精子数量和活力。同时，在出生后第49天，暴露组小鼠的精子异常率显著增加，但在出生后第152天与对照组相当。对处理动物睾丸的组织病理学分析显示，生精小管中生殖细胞脱落呈剂量依赖性增加。用10μg TBTCl/千克处理的小鼠在出生后第49天睾丸内17β-雌二醇（E2）水平降低，然后在出生后第152天明显恢复。而在处死时，对照组和TBTCl暴露组后代的血清E2、睾酮（T）水平以及睾丸内T水平之间未检测到显著差异。这些结果表明，围产期暴露于TBTCl与雄性生殖系统的长期改变有关，并且这些变化可能会持续到成年期。
