Department of Clinical Science at North Bristol, University of Bristol, UK.
Biomaterials. 2010 Mar;31(9):2583-91. doi: 10.1016/j.biomaterials.2009.12.023. Epub 2010 Jan 6.
Injuries to the avascular region of knee meniscal cartilage do not heal spontaneously. To address this problem we have developed a new stem cell/collagen-scaffold implant system in which human adult bone marrow mesenchymal stem cells are seeded onto a biodegradable scaffold that allows controlled delivery of actively dividing cells to the meniscus surface. Sandwich constructs of two white zone ovine meniscus discs with stem cell/collagen-scaffold implant in between were cultured in vitro for 40 days. Histomorphometric analysis revealed superior integration in the stem cell/collagen-scaffold groups compared to the cell-free collagen membrane or untreated controls. The addition of TGF-beta1 to differentiate stem cells to chondrocytes inhibited integration. Biomechanical testing demonstrated a significant 2-fold increase in tensile strength in all constructs using the stem cell/collagen-scaffold compared to control groups after 40 days in culture. Integration was significantly higher when collagen membranes were used that had a more open/spongy structure adjacent to both meniscal cartilage surfaces, whereas a collagen scaffold designed for osteoinduction failed to induce any integration of meniscus. In conclusion, the stem cell/collagen-scaffold implant is a potential therapeutic treatment for the repair of white zone meniscal cartilage tears.
膝关节半月板无血管区损伤不会自发愈合。为了解决这个问题，我们开发了一种新的干细胞/胶原支架植入系统，其中将人成体骨髓间充质干细胞接种到可生物降解的支架上，允许将活跃分裂的细胞受控递送到半月板表面。将两块白色区域绵羊半月板盘的三明治结构夹在中间的干细胞/胶原支架植入物在体外培养 40 天。组织形态计量学分析显示，与无细胞胶原膜或未处理对照相比，干细胞/胶原支架组的整合效果更好。添加 TGF-β1 将干细胞分化为软骨细胞会抑制整合。生物力学测试表明，与对照组相比，在培养 40 天后，所有使用干细胞/胶原支架的构建体的拉伸强度都显著增加了 2 倍。当胶原膜更靠近半月板软骨表面具有更开放/海绵状结构时，整合度更高，而设计用于诱导成骨的胶原支架未能诱导半月板的任何整合。总之，干细胞/胶原支架植入物是修复白色区域半月板软骨撕裂的潜在治疗方法。
