Cancer and Developmental Cell Biology Division, Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Singapore, Singapore.
Curr Opin Struct Biol. 2010 Feb;20(1):98-103. doi: 10.1016/j.sbi.2009.12.005. Epub 2010 Jan 5.
Translation termination is mediated by two classes of peptide release factors (RFs). Class I RF recognizes the stop codons and triggers the release of the polypeptide chain. Class II RF in prokaryotes promotes dissociation of class I RFs from the ribosome whereas in eukaryotes it acts cooperatively with class I RF to ensure efficient stop codon recognition and fast peptide release. Advances have been made in recent years at astonishing pace to determine the high-resolution crystal structures of both classes of RFs on and off the ribosome. Combined with biochemical analyses, these structures provide considerable insights into the mechanistic principles of stop codon recognition and peptide release.
翻译终止由两类肽释放因子（RFs）介导。I 类 RF 识别终止密码子并触发多肽链的释放。原核生物中的 II 类 RF 促进 I 类 RF 从核糖体上解离，而在真核生物中，它与 I 类 RF 协同作用，以确保有效的终止密码子识别和快速的肽释放。近年来，在确定核糖体上和核糖体外两类 RF 的高分辨率晶体结构方面取得了惊人的进展。这些结构与生化分析相结合，为终止密码子识别和肽释放的机制原理提供了重要的见解。
