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日本血吸虫重组蛋白用于血吸虫病诊断的比较

Comparison of Recombinant Proteins from Schistosoma japonicum for Schistosomiasis Diagnosis.

作者信息

Jin Ya-mei, Lu Ke, Zhou Wei-Fang, Fu Zhi-Qiang, Liu Jin-Ming, Shi Yao-Jun, Li Hao, Lin Jiao-Jiao

机构信息

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, People's Republic of China.

出版信息

Clin Vaccine Immunol. 2010 Mar;17(3):476-80. doi: 10.1128/CVI.00418-09. Epub 2010 Jan 6.

Abstract

The most important animal reservoirs of Schistosoma japonicum in China are bovines. Diagnosis and control of bovine schistosomiasis is critical for reducing the prevalence of the disease. We screened defined diagnostic antigens that have the potential to increase the sensitivity and specificity of serological assays and to distinguish between active and prior infections. Five recombinant proteins with the potential to be diagnostic antigens were compared to the native soluble egg antigen preparation by enzyme-linked immunosorbent assay (ELISA). We evaluated the potentials of the recombinant proteins for discriminating active from prior infections, as well as the therapeutic efficacy of the established ELISA technique.

摘要

在中国,日本血吸虫最重要的动物储存宿主是牛。牛血吸虫病的诊断和控制对于降低该病的流行率至关重要。我们筛选了特定的诊断抗原,这些抗原有可能提高血清学检测的敏感性和特异性,并区分现症感染和既往感染。通过酶联免疫吸附测定(ELISA),将五种具有诊断抗原潜力的重组蛋白与天然可溶性虫卵抗体制剂进行了比较。我们评估了重组蛋白区分现症感染和既往感染的潜力,以及既定ELISA技术的治疗效果。

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