First Department of Internal Medicine, Johannes Gutenberg University of Mainz, Mainz, Germany.
Int J Colorectal Dis. 2010 Apr;25(4):417-24. doi: 10.1007/s00384-009-0868-y.
Chemokines and their receptors have been proposed to distinctly contribute to tumor growth, dissemination, and local immune escape. The aim of this study was to evaluate the relevance of the chemokine receptor CCR5 expression for the progression of human colorectal cancer.
CCR5 expression was assessed by RT-PCR analysis in 103 colorectal cancer patients. Intensity of CCR5 expression was correlated with both tumor and patient characteristics. Infiltration of tumor margins with CD8(+) T cells in the context of CCR5 expression was analyzed by immunohistochemistry in additional 18 colorectal cancer specimens.
Human colorectal cancer revealed variable intensities of CCR5 expression ranging from absent (48/103: 47%), weak (30/103: 29%), intermediate (13/103: 13%), to strong (12/103: 12%). Absent or weak CCR5 expression was significantly associated with advanced UICC stages (P=0.02) and lymphatic metastasis (P=0.05). In addition, CCR5 expression positively correlated with CD8(+) T-cell infiltration in tumor margins (P=0.001).
In summary, intermediate and strong CCR5 expression was significantly associated with nonmetastatic colorectal cancer and increased CD8(+) T-cell infiltration.
趋化因子及其受体被认为对肿瘤的生长、扩散和局部免疫逃逸有独特的贡献。本研究旨在评估趋化因子受体 CCR5 的表达与人类结直肠癌进展的相关性。
通过 RT-PCR 分析在 103 例结直肠癌患者中评估 CCR5 表达。CCR5 表达的强度与肿瘤和患者特征相关联。在另外 18 例结直肠癌标本中，通过免疫组织化学分析 CCR5 表达背景下肿瘤边缘的 CD8(+) T 细胞浸润情况。
人结直肠癌的 CCR5 表达强度从缺失（48/103：47%）、弱（30/103：29%）、中等（13/103：13%）到强（12/103：12%）不等。缺失或弱 CCR5 表达与 UICC 晚期（P=0.02）和淋巴转移（P=0.05）显著相关。此外，CCR5 表达与肿瘤边缘的 CD8(+) T 细胞浸润呈正相关（P=0.001）。
总之，中等和强 CCR5 表达与非转移性结直肠癌显著相关，并增加了 CD8(+) T 细胞浸润。
