Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.
J Biomed Nanotechnol. 2009 Apr;5(2):215-23. doi: 10.1166/jbn.2009.1022.
The feasibility of using niobium oxide-coated magnetic nanoparticles (NPs) as affinity probes for selectively trapping phosphopeptides from peptide mixtures including tryptic digest of caseins, serum, and cell lysate was demonstrated in this study. Phosphopeptide enrichment was rapid when subjecting these systems to microwave heating for 1 min; the probe-target species, which were readily isolated through magnetic separation, were then analyzed using matrix-assisted laser desorption/ ionization mass spectrometry (MALDI MS). Only signals for phosphopeptides were present in the resulting mass spectra. The detection limit for monophosphopeptide was as low as 5 fmol.
本研究旨在证明氧化铌涂层磁性纳米粒子（NPs）作为亲和探针从包括酪蛋白、血清和细胞裂解物的胰蛋白酶消化物的肽混合物中选择性捕获磷酸肽的可行性。当将这些体系进行微波加热 1 分钟时，磷酸肽的富集非常迅速；然后通过磁分离很容易将探针-靶标物质分离出来，再使用基质辅助激光解吸/电离质谱（MALDI MS）进行分析。在得到的质谱中仅出现了磷酸肽的信号。单磷酸肽的检测限低至 5 fmol。
