U.S. Army Edgewood Chemical Biological Center, 5183 Black Hawk Road, Aberdeen Proving Ground, Maryland 21010-5424, USA.
Anal Chem. 2010 Feb 1;82(3):798-800. doi: 10.1021/ac9025972.
Biotoxin activity assays typically involve multistep sample preparation, multicomponent reactions, multistep analysis, or a combination thereof. We report a single-step, real-time ricin activity assay that requires little or no sample preparation and employs direct analysis in real time mass spectrometry. The release of adenine from the inhomogeneous substrate herring sperm DNA by ricin was determined to be 53 +/- 2 pmol adenine per picomole of ricin per hour. This procedure can be readily adapted to any enzyme for which a reactant or product of low molecular weight (up to approximately 600) can be identified.
生物毒素活性检测通常涉及多步样品制备、多组分反应、多步分析，或其组合。我们报告了一种单步、实时蓖麻毒素活性检测方法，它几乎不需要样品制备，并采用实时直接分析质谱法。通过蓖麻毒素从不均匀基质鲱鱼精子 DNA 中释放出的腺嘌呤被确定为每皮摩尔蓖麻毒素每小时释放 53±2 皮摩尔腺嘌呤。该程序可以很容易地适用于任何可以鉴定低分子量（约 600）反应物或产物的酶。
