Stanwick T L, Anderson R W, Nahmias A J
Infect Immun. 1977 Nov;18(2):342-7. doi: 10.1128/iai.18.2.342-347.1977.
Infection of human fibroblasts and HEp-2 cells with herpes simplex virus type 1 (HSV-1) produced a decrease in the intracellular levels of cyclic adenosine 5'- monophosphate (cAMP) and a concomitant increase in the cyclic guanosine 5'- monophosphate (cGMP) levels. In both cell cultures, changes in cyclic nucleotide levels were first observed at 6 h after viral inoculation and were maximal at 12 h. In human fibroblasts, the addition of theophylline, dibutyryl cAMP, or papaverine (cAMP-enhancing compounds) decreased significantly the yield of HSV-1, whereas the addition of insulin or dibutyryl cGMP (cGMP-enhancing compounds) increased the viral yield. In HEp-2 cells, only theophylline decreased the yield of HSV-1, and the cGMP-enhancing compounds had no apparent effect. Cyclic nucleotide enhancing compounds exhibited their effect only if added to either cell culture within the first 3 h after inoculation with HSV-1.
用1型单纯疱疹病毒（HSV-1）感染人成纤维细胞和HEp-2细胞，会导致细胞内环磷酸腺苷（cAMP）水平降低，同时环磷酸鸟苷（cGMP）水平升高。在两种细胞培养物中，病毒接种后6小时首次观察到环核苷酸水平的变化，12小时时达到最大值。在人成纤维细胞中，添加茶碱、二丁酰cAMP或罂粟碱（cAMP增强化合物）可显著降低HSV-1的产量，而添加胰岛素或二丁酰cGMP（cGMP增强化合物）则会增加病毒产量。在HEp-2细胞中，只有茶碱会降低HSV-1的产量，而cGMP增强化合物没有明显作用。环核苷酸增强化合物只有在接种HSV-1后的前3小时内添加到任一细胞培养物中才会发挥作用。
