Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo De Lellis s.n.c., 01100 Viterbo, Italy.
Bioresour Technol. 2010 May;101(9):3004-12. doi: 10.1016/j.biortech.2009.12.020. Epub 2010 Jan 6.
The ability of stationary and shaken Lentinus tigrinus CBS 577.79 liquid cultures to degrade a mixture of polycyclic aromatic hydrocarbons (PAHs) in N-rich (i.e., malt extract glucose, MEG) and in N-limited (low-N Kirk's medium, LNKM) media was investigated. Best results were obtained in shaken cultures where PAHs were degraded by 91% and 97% in MEG and LNKM, respectively; in stationary cultures, on the contrary, the degradation was never higher than 50%. Laccase activity was predominant on MEG while Mn-peroxidase (MnP) was preferentially produced in LNKM. The identification of degradation products showed the presence of several PAH derivatives, such as quinones, dicarboxylated and ring fission derivatives, presumably derived from the action of lignin-modifying enzymes. The presence of some degradation products (e.g., hydroxylated derivatives of anthrone and phenanthrene 9,10-dihydrodiol) suggested the possible involvement of cytochrome P-450-epoxide hydrolase system, the active form of which was found in 7-day-old cultures on MEG. In vitro experiments showed that the MnP from L. tigrinus had wider PAH substrate range and higher oxidation ability than the laccase produced by the same strain.
研究了富氮（即麦芽提取物葡萄糖，MEG）和贫氮（低氮柯克培养基，LNKM）培养基中静止和摇瓶培养的齿孔菌 CBS 577.79 液体对多环芳烃（PAHs）混合物的降解能力。在摇瓶培养中获得了最佳结果，MEG 和 LNKM 中 PAHs 的降解率分别为 91%和 97%；相反，在静止培养中，降解率从未高于 50%。MEG 中主要存在漆酶活性，而 LNKM 中优先产生锰过氧化物酶（MnP）。降解产物的鉴定表明存在多种 PAH 衍生物，如醌、二羧化和环裂衍生物，可能源自木质素修饰酶的作用。一些降解产物的存在（例如，蒽酮和菲 9,10-二氢二醇的羟基衍生物）表明可能涉及细胞色素 P-450-环氧化物水解酶系统，在 MEG 上培养 7 天时发现了其活性形式。体外实验表明，来自齿孔菌的 MnP 比同一菌株产生的漆酶具有更广泛的 PAH 底物范围和更高的氧化能力。
