Valizadeh Shiler, Rezaei Shahla, Mohamadnia Sonia, Rahimi Elaheh, Tavakoli Omid, Faramarzi Mohammad Ali
School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, 14176 Iran.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, 1417614411 Tehran, Iran.
J Environ Health Sci Eng. 2022 Feb 28;20(1):227-239. doi: 10.1007/s40201-021-00771-1. eCollection 2022 Jun.
Phenanthrene (Phe), a tricyclic Polycyclic Aromatic Hydrocarbon (PAH), is found in high concentrations as a pollutant in various environments. In this study, the removal or (oxidizing) ability of Phe by a laccase from was investigated. The laccase (12 U mL) was able to remove 63% of Phe (50 mg L) under optimal conditions of 40 °C, pH 8, 1.5 M NaCl and in the presence of 1 mM HBT as a laccase mediator after a 72 h incubation period. The results for the effect of different solvents, ionic and non-ionic surfactants on the activity of the halophilic laccase towards Phe showed that the addition of these compounds increase removal efficiency and complete enzymatic removal of Phe will achieve in a solution of 5% (v/v) acetone and 1.5% tween 80. The kinetic parameters and of laccase-catalyzed removal of the substrate were determined as 0.544 mM and 0.882 µmol h mg, respectively. A microtoxicity study with respect to the inhibition of algal growth showed a decrease in toxicity of the laccase-treated Phe solution.
菲（Phe）是一种三环多环芳烃（PAH），在各种环境中作为污染物以高浓度存在。在本研究中，研究了一种漆酶对菲的去除或（氧化）能力。在40℃、pH 8、1.5 M NaCl的最佳条件下，在1 mM HBT作为漆酶介体存在的情况下，经过72小时的孵育期后，漆酶（12 U/mL）能够去除63%的菲（50 mg/L）。不同溶剂、离子和非离子表面活性剂对嗜盐漆酶对菲的活性影响的结果表明，添加这些化合物可提高去除效率，在5%（v/v）丙酮和1.5%吐温80的溶液中可实现菲的完全酶促去除。漆酶催化去除底物的动力学参数Km和Vmax分别测定为0.544 mM和0.882 μmol h-1 mg-1。关于抑制藻类生长的微毒性研究表明，漆酶处理的菲溶液的毒性降低。
