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[香菇中木质素分解酶活性与磷脂酶活性之间的关系]

[Relation between ligninolytic and phospholipase activities in the fungus Lentinus tigrinus].

作者信息

Kadimaliev D A, Shutova V V, Teliatnik V I, Revin V V, Kezina E V, Kudaeva T V

出版信息

Mikrobiologiia. 2014 Jul-Aug;83(4):426-35.

PMID:25844453
Abstract

Effect of hydrocortisone, NaF, and FeSO4 on ligninolytic and phosphatase activity of the fungus Lentinus (Panus) tigrinus VKM F-3616D was investigated, Hydrocortisone and NaF were shown to inhibit the enzymes of the ligninolytic complex-laccase (EC 1.10.3.2), secretory peroxidase (EC 1.11.1.7), and Mn peroxidase (EC 1.11.1.13). FeSO4 exhibited no significant effect on the activity of these enzymes. Decreased activity of the enzymes of the ligninolytic complex was associatedwith inhibition of the activity and changes in the substrate specificity of phospholipase A2 (EC 3.1.1.4) in the presence of hydrocortisone of NaF. Cultivation of L. tigrinus in the presence of these compounds resulted in higher affinity of this enzyme to saturated fatty acids, while in the control and in the presence of FeSO4 affinity to unsaturated fatty acids was higher.

摘要

研究了氢化可的松、氟化钠和硫酸亚铁对真菌虎皮香菇(Lentinus (Panus) tigrinus VKM F - 3616D)木质素分解酶和磷酸酶活性的影响。结果表明,氢化可的松和氟化钠可抑制木质素分解复合酶系中的漆酶(EC 1.10.3.2)、分泌型过氧化物酶(EC 1.11.1.7)和锰过氧化物酶(EC 1.11.1.13)。硫酸亚铁对这些酶的活性无显著影响。在氢化可的松或氟化钠存在的情况下,木质素分解复合酶系中酶活性的降低与磷脂酶A2(EC 3.1.1.4)活性的抑制以及底物特异性的变化有关。在这些化合物存在的情况下培养虎皮香菇,该酶对饱和脂肪酸的亲和力更高,而在对照以及硫酸亚铁存在的情况下,对不饱和脂肪酸的亲和力更高。

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