来自水稻白叶枯病菌的DNA促旋酶GyrB亚基的结晶及初步X射线晶体学分析
Crystallization and preliminary X-ray crystallographic analysis of DNA gyrase GyrB subunit from Xanthomonas oryzae pv. oryzae.
作者信息
Jung Ha Yun, Lee Ki Jeung, Kim Kyung Ha, Hyoung Ji Hye, Han Mi Ra, Kim Hyun Kyoung, Kang Lin-Woo, Ahn Yeh-Jin, Heo Yong-Seok
机构信息
Department of Chemistry, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):48-50. doi: 10.1107/S1744309109047721. Epub 2009 Dec 25.
Abstract
DNA gyrase is a type II topoisomerase that is essential for chromosome segregation and cell division owing to its ability to modify the topological forms of bacterial DNA. In this study, the N-terminal fragment of the GyrB subunit of DNA gyrase from Xanthomonas oryzae pv. oryzae was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.10 A resolution using a synchrotron-radiation source. The crystal belonged to space group I4(1), with unit-cell parameters a = b = 110.27, c = 70.75 A. The asymmetric unit contained one molecule, with a V(M) of 2.57 A(3) Da(-1) and a solvent content of 50.2%.
摘要
DNA促旋酶是一种II型拓扑异构酶，由于其能够改变细菌DNA的拓扑形式，对于染色体分离和细胞分裂至关重要。在本研究中，来自水稻黄单胞菌稻瘟病菌株的DNA促旋酶GyrB亚基的N端片段在大肠杆菌中过表达、纯化并结晶。使用同步辐射源收集了分辨率为2.10 Å的衍射数据。晶体属于空间群I4(1)，晶胞参数a = b = 110.27，c = 70.75 Å。不对称单元包含一个分子，V(M)为2.57 ų Da⁻¹，溶剂含量为50.2%。
相似文献
1
Crystallization and preliminary X-ray crystallographic analysis of DNA gyrase GyrB subunit from Xanthomonas oryzae pv. oryzae.来自水稻白叶枯病菌的DNA促旋酶GyrB亚基的结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):48-50. doi: 10.1107/S1744309109047721. Epub 2009 Dec 25.
2
Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the C-terminal domain of the GyrA subunit of DNA gyrase from Staphylococcus aureus strain Mu50.金黄色葡萄球菌Mu50株DNA促旋酶GyrA亚基C末端结构域的过表达、纯化、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Feb 1;67(Pt 2):277-9. doi: 10.1107/S1744309110052188. Epub 2011 Jan 27.
3
Preliminary X-ray crystallographic analysis of the breakage-reunion domain of the GyrA subunit of DNA gyrase from Colwellia psychrerythraea strain 34H.来自嗜冷红杆菌34H菌株的DNA促旋酶GyrA亚基断裂-重连结构域的初步X射线晶体学分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):439-41. doi: 10.1107/S1744309110005567. Epub 2010 Mar 31.
4
Crystallization and X-ray diffraction data collection of topoisomerase IV ParE subunit from Xanthomonas oryzae pv. oryzae.来自水稻白叶枯病菌的拓扑异构酶IV ParE亚基的结晶及X射线衍射数据收集
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt 6):612-4. doi: 10.1107/S1744309109016649. Epub 2009 May 22.
5
Crystallization and preliminary X-ray crystallographic analysis of the XoGroEL chaperonin from Xanthomonas oryzae pv. oryzae.来自水稻黄单胞菌稻瘟病菌株的XoGroEL伴侣蛋白的结晶及初步X射线晶体学分析。
Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):604-7. doi: 10.1107/S2053230X14006591. Epub 2014 Apr 15.
6
Purification, crystallization and preliminary X-ray crystallographic studies of the Mycobacterium tuberculosis DNA gyrase CTD.结核分枝杆菌DNA解旋酶C端结构域的纯化、结晶及初步X射线晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):178-80. doi: 10.1107/S1744309111051888. Epub 2012 Jan 26.
7
Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of 3-dehydroquinate synthase, Xoo1243, from Xanthomonas oryzae pv. oryzae.来自水稻白叶枯病菌的3-脱氢奎尼酸合酶Xoo1243的克隆、表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt 12):1128-31. doi: 10.1107/S1744309108033575. Epub 2008 Nov 28.
8
Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine γ-synthase (XometB) from Xanthomonas oryzae pv. oryzae.来自水稻白叶枯病菌的胱硫醚γ-合酶（XometB）的表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1515-7. doi: 10.1107/S1744309112042819. Epub 2012 Nov 14.
9
Crystallization and preliminary crystallographic studies of CbsA, a secretory exoglucanase from Xanthomonas oryzae pv. oryzae.来自水稻黄单胞菌稻致病变种的分泌型外切葡聚糖酶CbsA的结晶及初步晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Oct 1;68(Pt 10):1191-4. doi: 10.1107/S1744309112034197. Epub 2012 Sep 26.
10
Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Xanthomonas oryzae pv. oryzae.来自水稻黄单胞菌稻瘟病菌株的肽脱甲酰基酶的表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1031-3. doi: 10.1107/S1744309108031631. Epub 2008 Oct 28.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Molecular analysis of the hrp gene cluster in Xanthomonas oryzae pathovar oryzae KACC10859.水稻白叶枯病菌KACC10859中hrp基因簇的分子分析
Microb Pathog. 2008 Jun;44(6):473-83. doi: 10.1016/j.micpath.2007.12.002. Epub 2007 Dec 23.
3
The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice.水稻白叶枯病菌稻黄单胞菌稻致病变种KACC10331的基因组序列。
Nucleic Acids Res. 2005 Jan 26;33(2):577-86. doi: 10.1093/nar/gki206. Print 2005.
4
Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase.大肠杆菌拓扑异构酶IV ParE亚基（24和43千道尔顿）的晶体结构：单个残基决定新生霉素对拓扑异构酶IV和DNA促旋酶效力的差异。
Antimicrob Agents Chemother. 2004 May;48(5):1856-64. doi: 10.1128/AAC.48.5.1856-1864.2004.
5
Alteration of Escherichia coli topoisomerase IV to novobiocin resistance.大肠杆菌拓扑异构酶IV对新生霉素耐药性的改变。
Antimicrob Agents Chemother. 2003 Mar;47(3):941-7. doi: 10.1128/AAC.47.3.941-947.2003.
6
The ATP-binding site of type II topoisomerases as a target for antibacterial drugs.II型拓扑异构酶的ATP结合位点作为抗菌药物的靶点。
Curr Top Med Chem. 2003;3(3):283-303. doi: 10.2174/1568026033452500.
7
Molecular determinants of disease and resistance in interactions of Xanthomonas oryzae pv. oryzae and rice.水稻白叶枯病菌与水稻互作中病害及抗性的分子决定因素
Microbes Infect. 2002 Nov;4(13):1361-7. doi: 10.1016/s1286-4579(02)00004-7.
8
An open conformation of the Thermus thermophilus gyrase B ATP-binding domain.嗜热栖热菌促旋酶B ATP结合结构域的开放构象。
J Biol Chem. 2002 May 24;277(21):18947-53. doi: 10.1074/jbc.M111740200. Epub 2002 Feb 15.
9
Dimerization of Escherichia coli DNA-gyrase B provides a structural mechanism for activating the ATPase catalytic center.大肠杆菌DNA促旋酶B的二聚化提供了一种激活ATP酶催化中心的结构机制。
J Biol Chem. 2000 Mar 31;275(13):9468-75. doi: 10.1074/jbc.275.13.9468.
10
Crystallography & NMR system: A new software suite for macromolecular structure determination.晶体学与核磁共振系统：用于大分子结构测定的新软件套件。
Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21. doi: 10.1107/s0907444998003254.
Zotero 插件