来自水稻白叶枯病菌的胱硫醚γ-合酶（XometB）的表达、结晶及初步X射线晶体学分析
Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine γ-synthase (XometB) from Xanthomonas oryzae pv. oryzae.
作者信息
Ngo Ho-Phuong-Thuy, Kim Jin-Kwang, Kim Seung-Hwan, Pham Tan-Viet, Tran Thi-Huyen, Nguyen Dinh-Duc, Kim Jeong-Gu, Chung Sumi, Ahn Yeh-Jin, Kang Lin-Woo
机构信息
Department of Advanced Technology Fusion, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1515-7. doi: 10.1107/S1744309112042819. Epub 2012 Nov 14.
Abstract
Cystathionine γ-synthase (CGS) catalyzes the first step in the transsulfuration pathway leading to the formation of cystathionine from O-succinylhomoserine and L-cysteine through a γ-replacement reaction. As an antibacterial drug target against Xanthomonas oryzae pv. oryzae (Xoo), CGS from Xoo (XometB) was cloned, expressed, purified and crystallized. The XometB crystal diffracted to 2.4 Å resolution and belonged to the tetragonal space group I4(1), with unit-cell parameters a=b=165.4, c=241.7 Å. There were four protomers in the asymmetric unit, with a corresponding solvent content of 73.9%.
摘要
胱硫醚γ-合酶（CGS）催化转硫途径的第一步反应，即通过γ-取代反应，由O-琥珀酰高丝氨酸和L-半胱氨酸生成胱硫醚。作为针对水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，简称Xoo）的抗菌药物靶点，对来自Xoo的CGS（XometB）进行了克隆、表达、纯化及结晶。XometB晶体的衍射分辨率为2.4 Å，属于四方晶系空间群I4(1)，晶胞参数a = b = 165.4，c = 241.7 Å。不对称单元中有四个原体，相应的溶剂含量为73.9%。
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