Liu Lei, Gu Li-Juan, Zhang Dong-Liang, Wang Zhen, Wang Chun-Yan, Li Zheng, Sung Chang-Keun
Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejon, Republic of Korea.
Biosci Biotechnol Biochem. 2010;74(1):96-100. doi: 10.1271/bbb.90596. Epub 2010 Jan 7.
In this study, rare ginsenoside Rf was transformed into 20(S)-protopanaxatriol (PPT(S)) by glycosidase from Aspergillus niger. By investing the reaction conditions, the optimal conditions were obtained, as follows: pH 5.0, temperature 55 degrees C, and substrate concentration 1.25 mmol/l. Under optimal conditions, PPT(S) (1.13 micromol) prepared from 1.25 mumol Rf showed a higher yield (90.4%). The enzymatic reaction was analyzed by reversed-phase HPLC, suggesting the transformation pathway: Rf-->Rh1(S)-->PPT(S).
在本研究中，黑曲霉糖苷酶将稀有皂苷Rf转化为20(S)-原人参三醇（PPT(S)）。通过研究反应条件，获得了如下最佳条件：pH 5.0、温度55℃、底物浓度1.25 mmol/L。在最佳条件下，由1.25 μmol Rf制备的PPT(S)（1.13 μmol）产率更高（90.4%）。通过反相高效液相色谱法分析酶促反应，表明其转化途径为：Rf→Rh1(S)→PPT(S)。
