用嗜热栖热菌β-葡萄糖苷酶对人参皂苷 Re、Rg1 和 Rf 进行酶转化生成人参皂苷 Rg2 和苷元 PPT。
Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using β-glucosidase from Thermotoga neapolitana.
机构信息
College of Food Science and Engineering, Jilin Agriculture University, Changchun, 130118, Jilin, China.
出版信息
Biotechnol Lett. 2019 May;41(4-5):613-623. doi: 10.1007/s10529-019-02665-7. Epub 2019 Apr 9.
OBJECTIVES
To enzymatically transform protopanaxatriol by using β-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.
RESULTS
Recombinant β-glucosidase was purified, which molecular weight was about 79.5 kDa. High levels of ginsenoside were obtained using the follow reaction conditions: 2 mg ml ginsenoside, 25 U ml enzyme, 85 °C, and pH 5.0. β-glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3 h under the given conditions, respectively. The enzyme created 1.66 mg ml Rg2 from Re with 553 mg l h, 0.85 mg ml, and 1.01 mg ml APPT from Rg1 and Rf with 283 and 316 mg l h APPT.
CONCLUSIONS
β-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf.
目的
利用来自 Thermotoga neapolitana（T. neapolitana）DSM 4359 的β-葡萄糖苷酶对原人参三醇进行酶转化。
结果
纯化了重组β-葡萄糖苷酶，其分子量约为 79.5 kDa。在以下反应条件下，使用 2 mg ml 人参皂苷、25 U ml 酶、85°C 和 pH 5.0，可获得高浓度的人参皂苷。β-葡萄糖苷酶在给定条件下分别将人参皂苷 Re 完全转化为 Rg2、Rf 和 Rg1 为 APPT，反应 3 小时后。该酶以 553 mg l h、0.85 mg ml 和 1.01 mg ml 的 APPT 从 Re 中产生 1.66 mg ml 的 Rg2，从 Rg1 和 Rf 中产生 283 和 316 mg l h 的 APPT。
结论
β-葡萄糖苷酶可用于高效、快速、低成本地从 Re 制备人参皂苷 Rg2，以及从人参皂苷 Rg1 和 Rf 制备 APPT。
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