Youn J K, Santillana M, Hue G, Barski G
Int J Cancer. 1977 Nov 15;20(5):792-7. doi: 10.1002/ijc.2910200519.
Evolution of virus expression in different lymphoid organs as well as in solid syngeneic tumors of mice inoculated with an MuLV was studied with the aid of in vitro XC co-culture technique. When normal adult mice of strain XLII were inoculated intraperitoneally with a cultured Rauscher virus (RC), the virus could be detected, 10 days after inoculation, only in bone marrow in small amounts and thereafter no virus could be found in any of the organs tested, including bone marrow, spleen, thymus, lymph node and kidney. However, when age- and sex-matched parallel mice bearing syngeneic subcutaneous non-viral tumors were inoculated similarly with the RC virus, the virus could be detected abundantly not only in bone marrow and spleen but also in tumors during the first 3 weeks and even 6 weeks after virus inoculation. Transitional decrease or disappearance of the virus was observed around the 25th-31st day in organs and tumors of the inoculated mice. When the tumor mass was removed from these mice by surgery, the virus disappeared rapidly and definitely from all the organs tested. The virus recovered from in vitro explanted and cultured tumors, taken from mice inoculated with the virus, induced typical lymphoid leukemia in BALB/c mice inoculated as newborns. However, from certain aspects (hypertrophy of the thymus and lymph nodes), this virus was different from the original RC virus.
借助体外XC共培养技术，研究了接种MuLV的小鼠不同淋巴器官以及实体同基因肿瘤中病毒表达的演变。当XLII品系的正常成年小鼠经腹腔接种培养的劳氏肉瘤病毒（RC）时，接种10天后仅在骨髓中检测到少量病毒，此后在包括骨髓、脾脏、胸腺、淋巴结和肾脏在内的任何测试器官中均未发现病毒。然而，当年龄和性别匹配的携带同基因皮下非病毒肿瘤的平行小鼠以同样方式接种RC病毒时，在病毒接种后的前3周甚至6周内，不仅在骨髓和脾脏中，而且在肿瘤中都能大量检测到病毒。在接种小鼠的器官和肿瘤中，在第25 - 31天左右观察到病毒的逐渐减少或消失。当通过手术从这些小鼠身上切除肿瘤块时，病毒迅速且确切地从所有测试器官中消失。从接种病毒的小鼠体外取出并培养的肿瘤中回收的病毒，在新生时接种的BALB/c小鼠中诱发了典型的淋巴细胞白血病。然而，从某些方面（胸腺和淋巴结肥大）来看，这种病毒与原始的RC病毒不同。
