Nagao M, Takatori T, Wu B, Terazawa K, Gotouda H, Akabane H, Inoue K, Shimizu M
Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Med Sci Law. 1991 Jan;31(1):61-4. doi: 10.1177/002580249103100111.
In order to observe the localization and dynamics of paraquat in lung and brain, immunohistochemical approaches were conducted by using animals. Experimental paraquat-poisoned rats were sacrificed 3 hours, 12 hours, 24 hours, 3 days, 7 days and 10 days after intravenous administration of paraquat (5 mg/kg). In lung tissues, paraquat was localized in histiocytes, walls of blood vessels and bronchiolar epithelial cells from 3 hours to 10 days after the paraquat exposure, and interstitial pulmonary fibrosis was being developed with time. On the other hand, in brain tissues, paraquat was localized only in capillary walls and glial cells but not observed in nerve cells 10 days after the administration of paraquat.
为观察百草枯在肺和脑中的定位及动态变化，采用动物进行免疫组化研究。对实验性百草枯中毒大鼠静脉注射百草枯（5毫克/千克）后3小时、12小时、24小时、3天、7天和10天处死。在肺组织中，百草枯暴露后3小时至10天定位在组织细胞、血管壁和细支气管上皮细胞中，且肺间质纤维化随时间发展。另一方面，在脑组织中，百草枯给药10天后仅定位在毛细血管壁和神经胶质细胞中，未在神经细胞中观察到。
