Good David M, Coon Joshua J
Department of Chemistry, University of Wisconsin, Madison, WI, USA.
Methods Mol Biol. 2009;566:277-91. doi: 10.1007/978-1-59745-562-6_18.
Hinging on the concept that extracellular proteins and polypeptides will provide information on the physiological state of specific organs, or even entire organisms, proteomic analysis of biological fluids for biomarker discovery has seen rapid expansion in recent years. Although multiple studies have had success using mass spectrometric analytical techniques for determination of proteins within a sample, inspection of naturally occurring species has been difficult, with most analyses using bottom-up methodology. We have applied a new fragmentation method, electron transfer dissociation (ETD), to this problem. We have previously illustrated the benefits to spectral quality and total identifications when using a combination of the complementary fragmentation techniques, ETD, and collision-activated dissociation, for analysis of naturally occurring proteins and polypeptides within biological fluids.
基于细胞外蛋白质和多肽将提供特定器官甚至整个生物体生理状态信息的概念，近年来，用于发现生物标志物的生物体液蛋白质组分析迅速发展。尽管多项研究已成功使用质谱分析技术测定样品中的蛋白质，但对天然存在的物种进行检测一直很困难，大多数分析采用自下而上的方法。我们已将一种新的裂解方法——电子转移裂解（ETD）应用于此问题。我们之前已经证明，当结合使用互补裂解技术ETD和碰撞激活裂解来分析生物体液中的天然存在的蛋白质和多肽时，对光谱质量和总鉴定结果都有好处。
