Cen Yu-Ke, Lin Jian-Guang, Wang You-Liang, Wang Jun-You, Liu Zhi-Qiang, Zheng Yu-Guo
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.
Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, China.
Front Bioeng Biotechnol. 2020 Mar 27;8:232. doi: 10.3389/fbioe.2020.00232. eCollection 2020.
In recent years, there has been a noticeable increase in research interests on the species, which includes prevalent plant pathogens and human pathogens, common microbial food contaminants and industrial microbes. Taken the advantage of gibberellin synthesis, succeed in being a prevalent plant pathogen. At the meanwhile, was utilized for industrial production of gibberellins, a group of extensively applied phytohormone. has been known for its outstanding performance in gibberellin production for almost 100 years. Research activities relate to this species has lasted for a very long period. The slow development in biological investigation of is largely due to the lack of efficient research technologies and molecular tools. During the past decade, technologies to analyze the molecular basis of host-pathogen interactions and metabolic regulations have been developed rapidly, especially on the aspects of genetic manipulation. At the meanwhile, the industrial fermentation technologies kept sustained development. In this article, we reviewed the currently available research tools/methods for research, focusing on the topics about genetic engineering and gibberellin production.
近年来，对该物种的研究兴趣显著增加，该物种包括常见的植物病原体和人类病原体、常见的微生物食品污染物以及工业微生物。利用赤霉素合成优势，成功成为一种常见的植物病原体。与此同时，它被用于赤霉素的工业生产，赤霉素是一类广泛应用的植物激素。在赤霉素生产方面，它已经有近100年的卓越表现。与该物种相关的研究活动持续了很长时间。对其生物学研究进展缓慢很大程度上是由于缺乏有效的研究技术和分子工具。在过去十年中，分析宿主 - 病原体相互作用和代谢调控分子基础的技术迅速发展，尤其是在基因操作方面。与此同时，工业发酵技术持续发展。在本文中，我们综述了目前可用于该研究的研究工具/方法，重点关注基因工程和赤霉素生产相关的主题。
