Sarov I, Abady I
Isr J Med Sci. 1977 Sep;13(9):887-95.
Human cytomegalovirions and dense bodies labeled with [14C]glucosamine were analyzed by means of sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis and autoradiography. The same glycopeptide composition was found in both cytomegalovirions and dense bodies. These consisted of at least 11 glycopeptides which ranged in molecular weight from 46,500 to over 170,000 daltons. Addition of purified human cytomegalovirus (CMV) at high multiplicity to confluent monolayers of human fibroblasts produced cell rounding and polykaryocytes containing 3 to 150 nuclei. The cell rounding was induced by the cytomegalovirions, but not by the cytomegalo dense bodies. Inhibition of protein synthesis, but not of DNA synthesis, prevented this effect, suggesting that cell rounding is protein mediated. In contrast, the formation of polykaryocytes by CMV was not affected by inhibitors of protein synthesis. UV irradiation of the virus, which abolishes infectivity, does not affect its fusion properties. CMV dense bodies, which contain very little or no DNA, also produce cell fusion although this effect is less pronounced than with virions. CMV-induced polykaryocytosis therefore appears to be a direct result of the interaction of cells with the input viral particles, a phenomenon usually referred to as early polykaryocytosis.
用十二烷基硫酸钠 - 尿素聚丙烯酰胺凝胶电泳和放射自显影法分析了用[¹⁴C]葡糖胺标记的人巨细胞病毒颗粒和致密体。在巨细胞病毒颗粒和致密体中发现了相同的糖肽组成。这些糖肽至少由11种糖肽组成，分子量范围从46,500到超过170,000道尔顿。以高感染复数向汇合的人成纤维细胞单层中加入纯化的人巨细胞病毒（CMV）会导致细胞变圆，并产生含有3至150个核的多核细胞。细胞变圆是由巨细胞病毒颗粒诱导的，而不是由巨细胞致密体诱导的。蛋白质合成的抑制而非DNA合成的抑制可阻止这种效应，这表明细胞变圆是由蛋白质介导的。相反，CMV诱导的多核细胞形成不受蛋白质合成抑制剂的影响。病毒的紫外线照射会消除其感染性，但不影响其融合特性。含有极少或不含DNA的CMV致密体也会产生细胞融合，尽管这种效应不如病毒颗粒明显。因此，CMV诱导的多核细胞增多似乎是细胞与输入病毒颗粒相互作用的直接结果，这一现象通常称为早期多核细胞增多。
