Department of Forensic Medicine, Wakayama Medical University, Wakayama, Japan.
Forensic Sci Int. 2010 Feb 25;195(1-3):143-7. doi: 10.1016/j.forsciint.2009.12.008. Epub 2010 Jan 8.
Using histochemical and immunohistochemical techniques, we examined the intrathrombotic collagen contents and the appearance of hemosiderin-positive cells, neovessels, and myofibroblasts in a stasis-induced venous thrombosis model. The intrathrombotic collagen deposition area occupied about 20% at 5 days, and exceeded 80% at 21 days after ligation of the inferior vena cava (IVC). Hemosiderin-positive cells in the thrombus first appeared at 3 days in only one of the five samples, and positive cells were constantly detected in all thrombi at 5 days or later. CD31-positive neovessels in the thrombus first appeared at 5 days in one of five samples and were detected in all samples after 10 days. At 7 days, alphaSMA-positive myofibroblasts at the periphery of the thrombus first appeared in three of five samples, and were detected and enhanced time-dependently in all samples after 10 days. These observations demonstrated that these markers would be applicable for thrombus age determination.
采用组织化学和免疫组织化学技术，我们在静脉淤滞性血栓形成模型中检测了血栓内胶原含量和含铁血黄素阳性细胞、新生血管和肌成纤维细胞的出现。在结扎下腔静脉（IVC）后 5 天，血栓内胶原沉积面积约占 20%，21 天后超过 80%。血栓内的含铁血黄素阳性细胞仅在 5 个样本中的一个中于第 3 天首次出现，并且在 5 天或之后的所有血栓中均持续检测到阳性细胞。血栓内的 CD31 阳性新生血管在 5 个样本中的一个中于第 5 天首次出现，在 10 天后所有样本中均有检测到。在第 7 天，血栓外周的 alphaSMA 阳性肌成纤维细胞在 5 个样本中的 3 个中首次出现，并且在 10 天后所有样本中均有检测到并随时间增强。这些观察结果表明，这些标志物可用于血栓形成时间的确定。
