Nosaka Mizuho, Ishida Yuko, Kimura Akihiko, Hama Mizuki, Kawaguchi Takashi, Yamamoto Hiroki, Kuninaka Yumi, Shimada Emi, Kondo Toshikazu
Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama, 641-8509, Japan.
Int J Legal Med. 2015 Sep;129(5):1021-5. doi: 10.1007/s00414-015-1147-9. Epub 2015 Jan 24.
We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-6(+) cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-6(+) cells. Thereafter, the numbers of IL-6(+) cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi.
我们使用下腔静脉（IVC）结扎诱导的深静脉血栓形成小鼠模型，对血栓内白细胞介素-6（IL-6）的表达进行了免疫组织化学检测，并探讨了血栓内IL-6在估计血栓年龄方面的可用性。在五个样本中的三个样本中，IVC结扎后1天首次检测到IL-6（+）细胞，所有结扎后间隔3天或更长时间的样本均有血栓内IL-6（+）细胞。此后，随着结扎后间隔时间的增加，IL-6（+）细胞数量升高。双色免疫荧光分析表明，IL-6主要由血栓内巨噬细胞表达。在所有结扎后间隔5天或更短时间的样本中，IL-6/巨噬细胞比值（IL-6/Mϕ）<0.5。相比之下，IVC结扎后≥7天时IL-6/Mϕ大于0.5。这些观察结果表明，IL-6/Mϕ比值>0.5强烈提示血栓年龄≥7天。相反，比值小于0.5提示血栓年龄≤5天。本研究表明，IL-6的免疫组织化学检测适用于估计静脉血栓的年龄。
