Scuola Superiore Sant'Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy.
Colloids Surf B Biointerfaces. 2010 Apr 1;76(2):535-43. doi: 10.1016/j.colsurfb.2009.12.015. Epub 2009 Dec 24.
Nanoscale structures and materials have been explored in many biological applications because of their extraordinary novel properties. Here we propose a study of cellular interactions with barium titanate nanoparticles, an interesting ceramic material that has received a lot of interest in the nanotechnology research, but without any attention about its biological potential. We introduced for the first time an efficient method for the preparation of stable aqueous dispersions of barium titanate nanoparticles, characterized with FIB, TEM and AFM imaging, light scattering, Z-potential and UV/vis analysis. Finally, we presented a systematic study of short-term cytotoxicity of the prepared dispersion based both on quantitative (metabolism, proliferation) and qualitative (apoptosis, viability, differentiation) assays.
由于其非凡的新颖特性，纳米结构和材料在许多生物应用中得到了探索。在这里，我们提出了一项关于细胞与钛酸钡纳米粒子相互作用的研究，钛酸钡是一种有趣的陶瓷材料，在纳米技术研究中受到了广泛关注，但关于其生物潜力的研究却很少。我们首次提出了一种制备稳定的钛酸钡纳米粒子水基分散体的有效方法，该方法采用 FIB、TEM 和 AFM 成像、光散射、Zeta 电位和紫外/可见分析进行了表征。最后，我们根据定量（代谢、增殖）和定性（凋亡、活力、分化）检测，系统地研究了所制备分散体的短期细胞毒性。
