Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera (Pisa), Italy.
Colloids Surf B Biointerfaces. 2013 Feb 1;102:312-20. doi: 10.1016/j.colsurfb.2012.08.001. Epub 2012 Aug 11.
Nanomaterials hold great promise in the manipulation and treatments of mesenchymal stem cells, since they allow the modulation of their properties and differentiation. However, systematic studies have to be carried out in order to assess their potential toxicological effects. The present study reports on biocompatibility evaluation of glycol-chitosan coated barium titanate nanoparticles (BTNPs) on rat mesenchymal stem cells (MSCs). BTNPs are a class of ceramic systems which possess interesting features for biological applications thanks to their peculiar dielectric and piezoelectric properties. Viability was evaluated up to 5 days of incubation (concentrations in the range 0-100 μg/ml) both quantitatively and qualitatively with specific assays. Interactions cells/nanoparticles were further investigated with analysis of the cytoskeleton conformation, with SEM and TEM imaging, and with AFM analysis. Finally, differentiation in adipocytes and osteocytes was achieved in the presence of high doses of BTNPs, thus highlighting the safety of these nanostructures towards mesenchymal stem cells.
纳米材料在间充质干细胞的操作和处理中具有很大的应用前景，因为它们可以调节其性质和分化。然而，为了评估它们潜在的毒理学效应，必须进行系统的研究。本研究报告了涂有乙二醇壳聚糖的钡钛酸盐纳米粒子（BTNPs）对大鼠间充质干细胞（MSCs）的生物相容性评价。BTNPs 是一类陶瓷体系，由于其独特的介电和压电特性，具有有趣的生物应用特性。通过特定的检测方法，在 5 天的孵育时间内（浓度范围为 0-100μg/ml）对细胞活力进行了定量和定性评估。通过分析细胞骨架构象、SEM 和 TEM 成像以及 AFM 分析，进一步研究了细胞/纳米粒子的相互作用。最后，在高剂量 BTNPs 的存在下实现了脂肪细胞和成骨细胞的分化，从而突出了这些纳米结构对间充质干细胞的安全性。
