Jordan Tomas, O'Brien Mikaela A, Spatarelu Catalina-Paula, Luke Geoffrey P
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States.
Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States; Translational Engineering in Cancer Research Program, Norris Cotton Cancer Center, Lebanon, New Hampshire 03766, United States.
ACS Appl Nano Mater. 2020 Mar 27;3(3):2636-2646. doi: 10.1021/acsanm.0c00019. Epub 2020 Mar 5.
Barium titanate nanoparticles (BTNPs) are gaining popularity in biomedical research because of their piezoelectricity, nonlinear optical properties, and high biocompatibility. However, the potential of BTNPs is limited by the ability to create stable nanoparticle dispersions in water and physiological media. In this work, we report a method of surface modification of BTNPs based on surface hydroxylation followed by covalent attachment of hydrophilic poly(ethylene glycol) (PEG) polymers. This polymer coating allows for additional modifications such as fluorescent labeling, surface charge tuning, or directional conjugation of IgG antibodies. We demonstrate the conjugation of anti-EGFR antibodies to the BTNP surface and show efficient molecular targeting of the nanoparticles to A431 cells. Overall, the reported modifications aim to expand the BTNP applications in molecular imaging, cancer therapy, or noninvasive neurostimulation.
钛酸钡纳米颗粒（BTNPs）因其压电性、非线性光学特性和高生物相容性而在生物医学研究中越来越受到关注。然而，BTNPs的应用潜力受到其在水和生理介质中形成稳定纳米颗粒分散体能力的限制。在本研究中，我们报道了一种基于表面羟基化，随后通过亲水性聚乙二醇（PEG）聚合物共价连接对BTNPs进行表面修饰的方法。这种聚合物涂层允许进行额外的修饰，如荧光标记、表面电荷调节或IgG抗体的定向偶联。我们展示了抗表皮生长因子受体（EGFR）抗体与BTNP表面的偶联，并表明纳米颗粒能够有效地靶向A431细胞。总体而言，所报道的修饰旨在扩大BTNPs在分子成像、癌症治疗或无创神经刺激方面的应用。
