SWIFT 技术在自上而下蛋白质组学中的高质量选择性。
High mass selectivity for top-down proteomics by application of SWIFT technology.
机构信息
Mass Spectrometry Facility and Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94158-2517, USA.
出版信息
J Am Soc Mass Spectrom. 2010 Mar;21(3):455-9. doi: 10.1016/j.jasms.2009.11.011. Epub 2009 Dec 11.
Abstract
Stored waveform inverse Fourier transform (SWIFT) technology has been implemented on a commercial Fourier transform ICR mass spectrometer. Complex ejection/isolation waveforms can be generated on an arbitrary waveform generator (AWG) and applied to the instrument by use of a high speed analog switch. High mass selectivity and subsequent electron capture dissociation (ECD) of the SWIFT isolated ions has been demonstrated with analysis of intact Bovine histone H4. A mass selectivity about 0.1 m/z unit for isolation of the 18+ charge state ions was achieved. Adaptation of SWIFT on the commercial instrument provides an enhanced capability for characterizing intact proteins by top-down analysis.
摘要
存储波形傅里叶逆变换（SWIFT）技术已在商用傅里叶变换离子回旋共振质谱仪上实现。复杂的逐出/隔离波形可以在任意波形发生器（AWG）上生成，并通过高速模拟开关应用于仪器。通过对完整的牛组蛋白 H4 的分析，证明了 SWIFT 隔离离子的高质量选择性和随后的电子俘获解离（ECD）。实现了对 18+电荷态离子的约 0.1 m/z 单位的质量选择性隔离。在商用仪器上对 SWIFT 的适配提供了通过自上而下分析对完整蛋白质进行特征分析的增强能力。
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