傅里叶变换质谱法。
Fourier transform mass spectrometry.
机构信息
Thermo Fisher Scientific, Bremen, Germany.
出版信息
Mol Cell Proteomics. 2011 Jul;10(7):M111.009431. doi: 10.1074/mcp.M111.009431.
Abstract
This article provides an introduction to Fourier transform-based mass spectrometry. The key performance characteristics of Fourier transform-based mass spectrometry, mass accuracy and resolution, are presented in the view of how they impact the interpretation of measurements in proteomic applications. The theory and principles of operation of two types of mass analyzer, Fourier transform ion cyclotron resonance and Orbitrap, are described. Major benefits as well as limitations of Fourier transform-based mass spectrometry technology are discussed in the context of practical sample analysis, and illustrated with examples included as figures in this text and in the accompanying slide set. Comparisons highlighting the performance differences between the two mass analyzers are made where deemed useful in assisting the user with choosing the most appropriate technology for an application. Recent developments of these high-performing mass spectrometers are mentioned to provide a future outlook.
摘要
这篇文章介绍了基于傅里叶变换的质谱法。从傅里叶变换离子回旋共振和轨道阱两种质谱仪的理论和工作原理的角度，阐述了基于傅里叶变换的质谱法的关键性能特征，即质量精度和分辨率，以及它们如何影响蛋白质组学应用中测量结果的解释。讨论了基于傅里叶变换的质谱技术在实际样品分析中的主要优点和局限性，并通过本文中的实例以及相关幻灯片进行了说明。在有助于用户选择最适合应用的技术的情况下，对两种质谱仪的性能差异进行了比较。提到了这些高性能质谱仪的最新发展，以提供未来展望。
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