CD9 蛋白表达下调与口腔鳞状细胞癌的侵袭行为有关。

Downregulation of CD9 protein expression is associated with aggressive behavior of oral squamous cell carcinoma.

机构信息

Department of Anatomical Pathology, Hospital A.C. Camargo, São Paulo, Brazil.

出版信息

Oral Oncol. 2010 Mar;46(3):166-71. doi: 10.1016/j.oraloncology.2009.11.009. Epub 2010 Jan 8.

DOI:10.1016/j.oraloncology.2009.11.009

PMID:20060356

Abstract

Squamous cell carcinoma of the oral cavity (OSCC) is a malignancy characterized by a high degree of local aggression and metastasis to cervical lymph nodes. Tetraspanins are proteins with functional roles in a wide array of cellular processes and are reported to be associated with tumor progression. The present study investigated the expression of the CD9, CD37, CD63, CD81 and CD82 tetraspanins in OSCC using immunohistochemistry (IHC) and quantitative Real Time-PCR (qRT-PCR). Tissue microarray (TMA) analysis of samples from 179 cases of OSCC and 10 normal samples oral mucosa were evaluated immunomorphologically. We analyzed CD9 and CD82 expression by qRT-PCR in 66 OSCC cases and 4 normal samples of oral mucosa. Expression of CD63, CD37 and CD81 was not detected in the samples studied. CD82 was downregulated or negative in 127 of 179 (80%) specimens; no correlation was observed between CD82 expression, clinicopathological parameters, disease-free survival and 5-year overall survival. CD9 expression was downregulated or negative in 75 of 129 (42%) OSCC samples. Loss of CD9 expression in OSCC samples correlated with the incidence of lymph node metastasis (p=0.017). Disease-free survival and the 5-year overall survival of patients with downregulated or negative CD9 expression were significantly lower than in patients with positive CD9 expression (p=0.010 and p=0.071, respectively). No correlation was found between CD9 or CD82 expression and clinicopathological parameters by qRT-PCR. Our results suggest that the downregulation or lack of expression of the CD9 protein might indicate a more aggressive of OSCC.

摘要

口腔鳞状细胞癌（OSCC）是一种恶性肿瘤，其特征为局部侵袭性高，并向颈部淋巴结转移。四跨膜蛋白在广泛的细胞过程中具有功能作用，并被报道与肿瘤进展有关。本研究采用免疫组织化学（IHC）和实时定量 PCR（qRT-PCR）检测 179 例 OSCC 组织和 10 例正常口腔黏膜组织中 CD9、CD37、CD63、CD81 和 CD82 四跨膜蛋白的表达。对 179 例 OSCC 病例和 10 例正常口腔黏膜组织的组织微阵列（TMA）进行免疫形态学评估。我们通过 qRT-PCR 分析了 66 例 OSCC 病例和 4 例正常口腔黏膜组织中 CD9 和 CD82 的表达。在研究的样本中未检测到 CD63、CD37 和 CD81 的表达。在 179 例标本中，CD82 下调或阴性表达 127 例（80%）；CD82 表达与临床病理参数、无病生存率和 5 年总生存率之间无相关性。在 129 例 OSCC 样本中，CD9 表达下调或阴性表达 75 例（42%）。OSCC 样本中 CD9 表达缺失与淋巴结转移的发生率相关（p=0.017）。CD9 表达下调或阴性的患者无病生存率和 5 年总生存率明显低于 CD9 表达阳性的患者（p=0.010 和 p=0.071）。通过 qRT-PCR 未发现 CD9 或 CD82 表达与临床病理参数之间的相关性。我们的研究结果表明，CD9 蛋白的下调或缺失可能预示着 OSCC 的侵袭性更强。