跨膜蛋白 CD82 与口腔癌中 S100A7 的表达相关，并可能对其进行调节。

Tetraspanin CD82 Correlates with and May Regulate S100A7 Expression in Oral Cancer.

机构信息

Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, 875 Union Ave, Memphis, TN 38163, USA.

Department of Pediatrics, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

出版信息

Int J Mol Sci. 2024 Feb 24;25(5):2659. doi: 10.3390/ijms25052659.

DOI:10.3390/ijms25052659

PMID:38473906

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10932236/

Abstract

Many metastatic cancers with poor prognoses correlate to downregulated CD82, but exceptions exist. Understanding the context of this correlation is essential to CD82 as a prognostic biomarker and therapeutic target. Oral squamous cell carcinoma (OSCC) constitutes over 90% of oral cancer. We aimed to uncover the function and mechanism of CD82 in OSCC. We investigated CD82 in human OSCC cell lines, tissues, and healthy controls using the CRISPR-Cas9 gene knockout, transcriptomics, proteomics, etc. CD82 expression is elevated in CAL 27 cells. Knockout CD82 altered over 300 genes and proteins and inhibited cell migration. Furthermore, CD82 expression correlates with S100 proteins in CAL 27, CD82KO, SCC-25, and S-G cells and some OSCC tissues. The 37-50 kDa CD82 protein in CAL 27 cells is upregulated, glycosylated, and truncated. CD82 correlates with S100 proteins and may regulate their expression and cell migration. The truncated CD82 explains the invasive metastasis and poor outcome of the CAL 27 donor. OSCC with upregulated truncated CD82 and S100A7 may represent a distinct subtype with a poor prognosis. Differing alternatives from wild-type CD82 may elucidate the contradictory functions and pave the way for CD82 as a prognostic biomarker and therapeutic target.

摘要

许多预后不良的转移性癌症与 CD82 的下调相关，但也存在例外。了解这种相关性的背景对于 CD82 作为预后生物标志物和治疗靶点至关重要。口腔鳞状细胞癌（OSCC）构成了超过 90%的口腔癌。我们旨在揭示 CD82 在 OSCC 中的功能和机制。我们使用 CRISPR-Cas9 基因敲除、转录组学、蛋白质组学等方法研究了人类 OSCC 细胞系、组织和健康对照中的 CD82。CD82 在 CAL 27 细胞中表达上调。敲除 CD82 改变了超过 300 个基因和蛋白质，并抑制了细胞迁移。此外，CD82 的表达与 CAL 27、CD82KO、SCC-25 和 S-G 细胞以及一些 OSCC 组织中的 S100 蛋白相关。CAL 27 细胞中的 37-50 kDa CD82 蛋白上调、糖基化和截断。CD82 与 S100 蛋白相关，可能调节其表达和细胞迁移。截断的 CD82 解释了 CAL 27 供体的侵袭性转移和不良预后。上调截断的 CD82 和 S100A7 的 OSCC 可能代表预后不良的独特亚型。来自野生型 CD82 的不同替代方案可能阐明了矛盾的功能，并为 CD82 作为预后生物标志物和治疗靶点铺平了道路。