单细胞测序揭示PHLDA1阳性平滑肌细胞促进头颈部鳞状细胞癌的局部侵袭。
Single-cell sequencing reveals PHLDA1-positive smooth muscle cells promote local invasion in head and neck squamous cell carcinoma.
作者信息
Guo Bing, Wen Xutao, Yu Shun, Yang Jun
机构信息
Department of Burns and Plastic Surgery, Institute of Traumatic Medicine and Department of Plastic Surgery and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, School of Medicine, College of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Engineering Research Center of Advanced Dental Technology and Materials, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Transl Oncol. 2025 May;55:102301. doi: 10.1016/j.tranon.2025.102301. Epub 2025 Mar 24.
BACKGROUND
Smooth muscle cells within the tumor microenvironment play a crucial role in cancer progression. However, their involvement in the local invasion of head and neck squamous cell carcinoma remains poorly understood. In this research, we aim to investigate the role of smooth muscle cells-mediated cell interactions in facilitating the local invasion of head and neck squamous cell carcinoma.
METHODS
Single-cell sequencing data from the public databases GSE164690 and GSE181919 were utilized to identify a specific smooth muscle cells cluster. Smooth muscle cells were isolated from tumor microenvironment of head and neck squamous cell carcinoma. PHLDA1 expression in smooth muscle cells was assessed through immunofluorescence staining. The role of THBS1 was investigated through in vitro studies.
RESULTS
PHLDA1-positive smooth muscle cells were significantly enriched in head and neck squamous cell carcinoma. PHLDA1 promoted the expression of THBS1 in smooth muscle cells. In vitro, THBS1 facilitated head and neck squamous cell carcinoma migration and invasion through SDC1 receptor.
CONCLUSION
PHLDA1-positive smooth muscle cells play a critical role in head and neck squamous cell carcinoma invasion through THBS1. Targeting PHLDA1-positive smooth muscle cells or THBS1 may offer a promising therapeutic approach for head and neck squamous cell carcinoma treatment.
背景
肿瘤微环境中的平滑肌细胞在癌症进展中起关键作用。然而，它们在头颈部鳞状细胞癌局部侵袭中的作用仍知之甚少。在本研究中，我们旨在探讨平滑肌细胞介导的细胞相互作用在促进头颈部鳞状细胞癌局部侵袭中的作用。
方法
利用来自公共数据库GSE164690和GSE181919的单细胞测序数据来鉴定特定的平滑肌细胞簇。从头颈部鳞状细胞癌的肿瘤微环境中分离平滑肌细胞。通过免疫荧光染色评估平滑肌细胞中PHLDA1的表达。通过体外研究探讨THBS1的作用。
结果
PHLDA1阳性平滑肌细胞在头颈部鳞状细胞癌中显著富集。PHLDA1促进平滑肌细胞中THBS1的表达。在体外，THBS1通过SDC1受体促进头颈部鳞状细胞癌的迁移和侵袭。
结论
PHLDA1阳性平滑肌细胞通过THBS1在头颈部鳞状细胞癌侵袭中起关键作用。靶向PHLDA1阳性平滑肌细胞或THBS1可能为头颈部鳞状细胞癌的治疗提供一种有前景的治疗方法。
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