直接红 81 和酰胺黑在聚丙烯酰胺电泳凝胶中 10 分钟内染色蛋白质。

Direct red 81 and amido black stain proteins in polyacrylamide electrophoresis gels within 10 min.

机构信息

Biochemistry, University of KwaZulu-Natal, P.B. X01 Scottsville 3209, South Africa.

出版信息

Anal Biochem. 2010 May 1;400(1):139-41. doi: 10.1016/j.ab.2009.12.038. Epub 2010 Jan 7.

DOI:10.1016/j.ab.2009.12.038

PMID:20060376

Abstract

Proteins separated by SDS-polyacrylamide gel electrophoresis can be stained with organic dyes, the most popular being Coomassie brilliant blue R-250. Coomassie R-250 staining of ovalbumin in an SDS-PAGE gel increased linearly from 2.5 to 60 min. Direct red 81 and amido black staining approached saturation in 10 min. Scatchard analysis showed that the number of direct red 81 and amido black ligands bound to ovalbumin was fourfold higher than that of Coomassie R-250. Direct red 81 and amido black stain proteins in an SDS-polyacrylamide electrophoresis gel in 10 min.

摘要

经 SDS-聚丙烯酰胺凝胶电泳分离的蛋白质可用有机染料染色，最常用的是考马斯亮蓝 R-250。考马斯亮蓝 R-250 染色 SDS-PAGE 凝胶中的卵清蛋白在 2.5 至 60 分钟内呈线性增加。直接红 81 和氨基黑染色在 10 分钟内接近饱和。Scatchard 分析表明，直接红 81 和氨基黑与卵清蛋白结合的配体数量是考马斯亮蓝 R-250 的四倍。直接红 81 和氨基黑在 10 分钟内可将 SDS-聚丙烯酰胺电泳凝胶中的蛋白质染色。