Saoji A M, Kulkarni M, Naghi T
Indian J Pathol Microbiol. 1989 Oct;32(4):286-90.
We hereby describe electrophoretic analysis of normal human serum pre-stained with Coomassie Brilliant Blue R-250 (CBB). The proposed method was optimized by studying as many as 450 disc electrophoretic separations and 30 variables. The method when compared with the post-electrophoretic staining by Amido Black (AB) revealed that the pre-stained discs were intensely well defined and resolved within 2 hours with a transparent gel. Gels stained with AB, despite a prolonged destaining, showed a residual dye retention making identification of the faint components difficult. Protein eluted from the CBB pre-stained gels retained its purity and immunoreactivity and conjugates of the two prototype proteins namely the albumin and the transferrin produced high-titre monospecific antisera in immunized rabbits.
我们在此描述了用考马斯亮蓝R - 250（CBB）预染的正常人血清的电泳分析。通过研究多达450次圆盘电泳分离和30个变量对所提出的方法进行了优化。与用氨基黑（AB）进行电泳后染色相比，该方法表明预染的圆盘在2小时内用透明凝胶清晰界定且分离良好。用AB染色的凝胶，尽管脱色时间延长，但仍有残留染料保留，使得难以鉴定微弱成分。从CBB预染凝胶中洗脱的蛋白质保持了其纯度和免疫反应性，两种原型蛋白质即白蛋白和转铁蛋白的缀合物在免疫兔中产生了高滴度的单特异性抗血清。
