Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.
Water Res. 2010 Apr;44(7):2292-302. doi: 10.1016/j.watres.2009.12.025. Epub 2009 Dec 21.
Soluble microbial products (SMP) are the pool of organic compounds originating from microbial growth and decay, and are usually the major component of the soluble organic matters in effluents from biological treatment processes. In this work, SMP in activated sludge were characterized, fractionized, and quantified using integrated chemical analysis and mathematical approach. The utilization-associated products (UAP) in SMP, produced in the substrate-utilization process, were found to be carbonaceous compounds with a molecular weight (MW) lower than 290 kDa which were quantified separately from biomass-associated products (BAP). The BAP were mainly cellular macromolecules with an MW in a range of 290-5000 kDa, and for the first time were further classified into the growth-associated BAP (GBAP) with an MW of 1000 kDa, which were produced in the microbial growth phase, and the endogeny-associated BAP (EBAP) with an MW of 4500 kDa, which were generated in the endogenous phase. Experimental and modeling results reveal that the UAP could be utilized by the activated sludge and that the BAP would accumulate in the system. The GBAP and EBAP had different formation rates from the hydrolysis of extracellular polymeric substances and distinct biodegradation kinetics. This study provides better understanding of SMP formation mechanisms and becomes useful for subsequent effluent treatment.
可溶解性微生物产物（SMP）是由微生物生长和衰亡所产生的有机化合物的集合，通常是生物处理过程中流出物中可溶解性有机物的主要成分。在这项工作中，我们采用综合化学分析和数学方法对活性污泥中的 SMP 进行了特征描述、分级和定量。在基质利用过程中产生的与利用相关的产物（UAP）是分子量（MW）低于 290 kDa 的碳质化合物，与生物量相关产物（BAP）是分开定量的。BAP 主要是分子量在 290-5000 kDa 范围内的细胞大分子，首次将其进一步分类为分子量为 1000 kDa 的与生长相关的 BAP（GBAP），它们是在微生物生长阶段产生的，以及分子量为 4500 kDa 的与内源相关的 BAP（EBAP），它们是在内源阶段产生的。实验和建模结果表明，UAP 可以被活性污泥利用，BAP 会在系统中积累。GBAP 和 EBAP 从细胞外聚合物的水解中具有不同的形成速率和不同的生物降解动力学。本研究更好地理解了 SMP 的形成机制，为后续的废水处理提供了帮助。
