巴西圈养食蟹狐（Cerdocyon thous）（食肉目，犬科）感染刚地弓形虫和利什曼原虫。

Toxoplasma gondii and Leishmania spp. infection in captive crab-eating foxes, Cerdocyon thous (Carnivora, Canidae) from Brazil.

机构信息

Instituto de Estudos Socioambientais do sul da Bahia (IESB), Rua Major Homem d'El Rey, 147 Cidade Nova, Ilhéus, BA, Brazil.

出版信息

Vet Parasitol. 2010 Apr 19;169(1-2):190-2. doi: 10.1016/j.vetpar.2009.12.019. Epub 2009 Dec 24.

DOI:10.1016/j.vetpar.2009.12.019

PMID:20060648

Abstract

The zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. Thus, the present study aimed to assess the prevalence of Leishmania spp. and Toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (Cerdocyon thous) kept in 17 zoos in São Paulo State, Brazil. Modified agglutination test (MAT, for toxoplasmosis) and indirect fluorescent antibody test (IFAT, for toxoplasmosis and leishmaniasis) were employed with heterologous anti-dog immunoglobulin. Antibodies to T. gondii were found in 19.2% animals, with an almost perfect concordance (kappa=0.86; standard error=9.31%; CI95%=68.25-104.76%; P<0.0001) and a strong correlation coefficient (rs=0.87; P<0.0001), which allows the use of heterologous anti-dog immunoglobulin to perform IFAT for toxoplasmosis in crab-eating foxes. No sample was positive for Leishmania spp. Toxoplasmosis infection occurs in wild animals from the studied Brazilian zoos, which indicates a probable environmental contamination, highlighting the importance of appropriate zoo management and the action of the parasite as a sentinel to human infection.

摘要

兽医学中的弓形虫病和利什曼病在全球范围内都很重要，也会影响野生动物。因此，本研究旨在评估巴西圣保罗州 17 个动物园中圈养的食蟹狐（Cerdocyon thous）52 份血清样本中利什曼原虫和弓形虫抗体的流行情况。采用改良凝集试验（MAT，用于检测弓形虫病）和间接荧光抗体试验（IFAT，用于检测弓形虫病和利什曼病），并使用异源性抗狗免疫球蛋白。结果发现，19.2%的动物存在弓形虫抗体，具有近乎完美的一致性（kappa=0.86；标准误差=9.31%；95%CI=68.25-104.76%；P<0.0001）和很强的相关系数（rs=0.87；P<0.0001），这允许使用异源性抗狗免疫球蛋白在食蟹狐中进行 IFAT 检测弓形虫病。没有样本对利什曼原虫呈阳性。来自研究中巴西动物园的野生动物感染了弓形虫病，这表明可能存在环境污染，突出了适当的动物园管理和寄生虫作为人类感染的哨兵的重要性。