Lignon Julia Somavilla, Pinto Diego Moscarelli, Monteiro Silvia Gonzalez, Martins Natália Soares, Martins Kauê Rodriguez, Dos Santos Tamires Silva, Meireles Giulia Ribeiro, de Albuquerque Luíse Nunes Bonneau, Cunha Rodrigo Casquero, Pappen Felipe Geraldo, Bruhn Fábio Raphael Pascoti
Laboratório de Epidemiologia Veterinária, Departamento de Veterinária Preventiva, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Laboratório do Grupo de Estudos em Enfermidades Parasitárias, Departamento de Veterinária Preventiva, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Braz J Vet Med. 2025 Jun 16;47:e000725. doi: 10.29374/2527-2179.bjvm000725. eCollection 2025.
Crab-eating fox () is widely distributed throughout South America, being the most common wild canid in Rio Grande do Sul, southern Brazil. It is considered a host for several parasites and contributes to the maintenance of its biological cycle due to its generalist and synanthropic habits. Due to the importance of the disease caused by , knowing its distribution in wild animals is essential to understand the transmission cycle of the protozoan. Therefore, the objective of the study was to report the molecular identification of DNA in a sample of cardiac muscle tissue from in southern Brazil. For this purpose, a specimen of , found dead after being run over, was collected on the highways of Cerrito, Rio Grande do Sul, Brazil and sent to the laboratory for necropsy. Tissue fragments (spleen, liver, kidney, heart, lung, lymph nodes, bone marrow and blood) were collected and its genomic DNA was extracted. The samples were subjected to polymerase chain reaction (PCR) amplification using the 18S rRNA gene, and DNA was amplified in cardiac muscle samples. The presence of the protozoan was confirmed by genetic sequencing. This study reports the molecular detection of DNA in cardiac muscle of in southern Brazil, demonstrating the presence of the protozoan in the studied region. In addition, a new molecular sequence is being provided, contributing to the knowledge and epidemiology of the parasite.
食蟹狐（）广泛分布于南美洲，是巴西南部南里奥格兰德州最常见的野生犬科动物。由于其具有泛食性和与人类共生的习性，它被认为是多种寄生虫的宿主，并有助于维持其生物循环。鉴于[病原体名称]引起的疾病的重要性，了解其在野生动物中的分布对于理解该原生动物的传播循环至关重要。因此，本研究的目的是报告在巴西南部食蟹狐心肌组织样本中[病原体名称]DNA的分子鉴定。为此，在巴西南里奥格兰德州塞里托的高速公路上收集了一只被车撞死后发现的食蟹狐标本，并送往实验室进行尸检。收集了组织碎片（脾脏、肝脏、肾脏、心脏、肺、淋巴结、骨髓和血液）并提取了其基因组DNA。使用18S rRNA基因对样本进行聚合酶链反应（PCR）扩增，在心肌样本中扩增出了[病原体名称]DNA。通过基因测序确认了原生动物的存在。本研究报告了在巴西南部食蟹狐心肌中[病原体名称]DNA的分子检测，证明了该原生动物在所研究地区的存在。此外，还提供了一个新的分子序列，有助于了解该寄生虫的知识和流行病学情况。
