Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
Vet Microbiol. 2010 Jul 14;143(2-4):126-32. doi: 10.1016/j.vetmic.2009.11.013. Epub 2009 Nov 18.
There are no reports of CDV isolations in southern Africa, and although CDV is said to have geographically distinct lineages, molecular information of African strains has not yet been documented. Viruses isolated in cell cultures were subjected to reverse transcription-polymerase chain reaction (RT-PCR), and the complete H gene was sequenced and phylogenetically analysed with other strains from GenBank. Phylogenetic comparisons of the complete H gene of CDV isolates from different parts of the world (available in GenBank) with wild-type South African isolates revealed nine clades. All South African isolates form a separate African clade of their own and thus are clearly separated from the American, European, Asian, Arctic and vaccine virus clades. It is likely that only the 'African lineage' of CDV may be circulating in South Africa currently, and the viruses isolated from dogs vaccinated against CDV are not the result of reversion to virulence of vaccine strains, but infection with wild-type strains.
目前尚无有关南部非洲犬瘟热病毒分离的报告，尽管犬瘟热病毒据称具有地理上不同的谱系，但尚未记录非洲毒株的分子信息。在细胞培养物中分离出的病毒进行逆转录聚合酶链反应（RT-PCR），并对完整的 H 基因进行测序，并与 GenBank 中的其他毒株进行系统发育分析。对来自世界不同地区的犬瘟热病毒分离株（可在 GenBank 中获得）的完整 H 基因与南非野生型分离株的系统发育比较显示出九个分支。所有南非分离株形成自己的单独的非洲分支，因此与美洲、欧洲、亚洲、北极和疫苗病毒分支明显分开。目前在南非循环的可能只有犬瘟热病毒的“非洲谱系”，而从接种犬瘟热疫苗的犬中分离出的病毒不是疫苗株毒力返祖的结果，而是感染了野生型毒株。
