School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW 2678, Australia.
Vet Microbiol. 2010 Jul 14;143(2-4):401-4. doi: 10.1016/j.vetmic.2009.11.042. Epub 2009 Dec 4.
There are currently two known serotypes of equine adenovirus (EAdV), equine adenovirus type 1 (EAdV1) and equine adenovirus type 2 (EAdV2); EAdV1 is predominantly associated with upper respiratory tract infections while EAdV2 appears to have a higher association with gastrointestinal infection, however, very little is known about the prevalence of these viruses in horse populations in Australia. In this study we tested 122 serum samples obtained from horses in New South Wales, Australia, using a standard serum neutralization (SN) assay and ELISA. Ninety-seven of the 122 sera displayed had moderate to high titers of antibodies to EAdV1 and/or EAdV2. Eighteen of the 122 sera were positive for both EAdV1 and EAdV2. In contrast, only thirty-seven positive samples were detected using the ELISA. These results suggest that EAdV1 and EAdV2 infections are widely prevalent in the horse population tested and that SN is currently the most suitable assay for the detection of EAdV1 and EAdV2 antibodies in equine serum.
目前已知有两种马腺病毒（EAdV）血清型，即马腺病毒 1 型（EAdV1）和马腺病毒 2 型（EAdV2）；EAdV1 主要与上呼吸道感染有关，而 EAdV2 似乎与胃肠道感染的相关性更高，但对于这些病毒在澳大利亚马群中的流行情况知之甚少。在这项研究中，我们使用标准血清中和（SN）测定法和 ELISA 检测了来自澳大利亚新南威尔士州的 122 份血清样本。在这 122 份血清中，有 97 份对 EAdV1 和/或 EAdV2 具有中等至高滴度的抗体。122 份血清中有 18 份对 EAdV1 和 EAdV2 均呈阳性。相比之下，ELISA 仅检测到 37 份阳性样本。这些结果表明，EAdV1 和 EAdV2 感染在被检测的马群中广泛流行，而 SN 目前是检测马血清中 EAdV1 和 EAdV2 抗体的最适合的检测方法。
