Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054, USA.
Curr Opin Struct Biol. 2010 Feb;20(1):114-20. doi: 10.1016/j.sbi.2009.12.006. Epub 2010 Jan 8.
Genome packaging is a fundamental process in a viral life cycle. Many viruses assemble preformed capsids into which the genomic material is subsequently packaged. These viruses use a packaging motor protein that is driven by the hydrolysis of ATP to condense the nucleic acids into a confined space. How these motor proteins package viral genomes had been poorly understood until recently, when a few X-ray crystal structures and cryo-electron microscopy (cryo-EM) structures became available. Here we discuss various aspects of genome packaging and compare the mechanisms proposed for packaging motors on the basis of structural information.
基因组包装是病毒生命周期中的一个基本过程。许多病毒将预先形成的衣壳组装在一起,随后将基因组物质包装进去。这些病毒使用一种包装马达蛋白,该蛋白通过 ATP 的水解来驱动,将核酸浓缩到一个受限的空间中。直到最近,当一些 X 射线晶体结构和低温电子显微镜(cryo-EM)结构可用时,这些马达蛋白如何包装病毒基因组才得到了很好的理解。在这里,我们讨论基因组包装的各个方面,并根据结构信息比较包装马达提出的机制。