Institute of Physiology and Neurophysiology Research Group of the Hungarian Academy of Sciences, Pécs University, Medical School, Szigeti str. 12, H-7624 Pécs, Hungary.
Appetite. 2010 Apr;54(2):354-62. doi: 10.1016/j.appet.2010.01.001. Epub 2010 Jan 11.
The present study investigates the effects of direct administration of interleukin-1beta (IL-1beta) into the nucleus accumbens (NAcc) on homeostatic regulation. Short- and long-term food intakes (FI), water intakes (WI) and body temperature (BT) were measured before and after bilateral microinjection of IL-1beta (with or without paracetamol pretreatment) into the NAcc of Wistar rats, and the effects were compared with those found in vehicle treated control animals. In addition, blood glucose levels, along with a glucose tolerance test (GTT), and plasma concentrations of metabolic parameters, such as total cholesterol, triglycerides, HDL, LDL and uric acid were determined in cytokine treated and control rats. Short-term FI and WI were suppressed after intraaccumbens application of IL-1beta. A significant increase of BT was also observed after the cytokine microinjection. Pretreatment with paracetamol failed to influence the anorexigenic, adipsogenic, and pyrogenic effects of IL-1beta. A definite glucose intolerance of the cytokine treated animals and their pathologically elevated blood glucose levels became obvious in the acute GTT. Following IL-1beta microinjection, plasma levels of triglycerides, total cholesterol and LDL were found increased. Our present findings show that the NAcc is an important site of action of IL-1beta mediated processes in central homeostatic regulation.
本研究探讨了直接向伏隔核（NAcc）内注射白细胞介素-1β（IL-1β）对体内平衡调节的影响。在 Wistar 大鼠双侧 NAcc 内注射 IL-1β（或预先给予对乙酰氨基酚）前后，测量了短期和长期食物摄入量（FI）、水摄入量（WI）和体温（BT），并将其与接受载体处理的对照动物的结果进行了比较。此外，在接受细胞因子治疗和对照大鼠中测定了血糖水平，以及葡萄糖耐量试验（GTT）和代谢参数（如总胆固醇、甘油三酯、HDL、LDL 和尿酸）的血浆浓度。向 NAcc 内注射 IL-1β 后，短期 FI 和 WI 受到抑制。细胞因子注射后，BT 也明显升高。预先给予对乙酰氨基酚不能影响 IL-1β 的厌食、致脂解和致热作用。在急性 GTT 中，明显观察到接受细胞因子治疗的动物存在明显的葡萄糖耐量异常和病理性高血糖。IL-1β 微注射后，发现血浆甘油三酯、总胆固醇和 LDL 水平升高。我们目前的研究结果表明，NAcc 是 IL-1β 介导的中枢体内平衡调节过程的重要作用部位。
