Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Acta Biomater. 2010 Jul;6(7):2540-7. doi: 10.1016/j.actbio.2010.01.004. Epub 2010 Jan 11.
Nanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300-500 microm paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young's modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications.
细菌生物合成的纳米多孔纤维素因其生物相容性和良好的机械性能而成为组织工程中一种有吸引力的生物材料支架。然而，对于骨骼应用，需要微观的孔结构来促进成骨细胞的向内生长和矿化组织的形成。因此，在这项研究中，通过将 300-500 微米的石蜡微球掺入发酵过程中，制备了微孔细菌纤维素（BC）支架。随后去除石蜡微球，扫描电子显微镜证实了支架的微孔表面，傅里叶变换红外光谱验证了石蜡的去除，拉伸测量表明杨氏模量约为 1.6 MPa。微孔 BC 和纳米孔（对照）BC 支架接种了 MC3T3-E1 成骨前体细胞，并通过共聚焦显微镜和组织学检查细胞分布和矿物质沉积情况。细胞在微孔 BC 的孔内聚集，并形成比在对照 BC 表面生长的细胞更密集的矿物质沉积物。这项工作表明，微孔 BC 是一种有前途的骨组织工程应用生物材料。
