Yodsanga Somchai, Poeaim Supattra, Chantarangsu Soranun, Swasdison Somporn
Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang (KMITL), Ladkrabang, Bangkok 10520, Thailand.
Department of Oral Pathology, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
Cells. 2025 May 15;14(10):723. doi: 10.3390/cells14100723.
Developing scaffolds with a three-dimensional porous structure and adequate mechanical properties remains a key challenge in tissue engineering of bone. These scaffolds must be biocompatible and biodegradable to effectively support osteoblastic cell attachment, metabolic activity, and differentiation. This study successfully fabricated a chitosan-bacterial cellulose (CS-BC) composite scaffold using the solvent casting/particle leaching (SCPL) technique, with NaOH/urea solution and sodium chloride crystals as the porogen. The scaffold exhibited a well-distributed porous network with pore sizes ranging from 300 to 500 µm. Biodegradation tests in PBS containing lysozyme revealed a continuous degradation process, while in vitro studies with MC3T3-E1 cells (pre-osteoblastic mouse cell line) demonstrated excellent cell attachment, as observed through SEM imaging. The scaffold also promoted increased metabolic activity (OD values) in the MTT assay, and enhanced alkaline phosphatase (ALP) activity and upregulated expression of osteogenic-related genes. These findings suggest that the CS-BC composite scaffold, fabricated using the SCPL method, holds great potential as a candidate for bone tissue engineering applications.
开发具有三维多孔结构和足够机械性能的支架仍然是骨组织工程中的关键挑战。这些支架必须具有生物相容性和可生物降解性，才能有效地支持成骨细胞的附着、代谢活性和分化。本研究采用溶剂浇铸/颗粒沥滤（SCPL）技术，以氢氧化钠/尿素溶液和氯化钠晶体为致孔剂，成功制备了壳聚糖-细菌纤维素（CS-BC）复合支架。该支架呈现出孔径范围为300至500μm的分布均匀的多孔网络。在含有溶菌酶的PBS中进行的生物降解测试显示出持续的降解过程，而对MC3T3-E1细胞（小鼠前成骨细胞系）的体外研究通过扫描电子显微镜成像观察到，细胞附着良好。在MTT试验中，该支架还促进了代谢活性（光密度值）的增加，并增强了碱性磷酸酶（ALP）活性，上调了成骨相关基因的表达。这些发现表明，采用SCPL方法制备的CS-BC复合支架作为骨组织工程应用的候选材料具有巨大潜力。
