Institute for Veterinary Bacteriology, Universität Bern, Länggassstrasse 122, Postfach, CH-3001 Bern, Switzerland.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2753-2757. doi: 10.1099/ijs.0.011692-0. Epub 2010 Jan 8.
Gram-negative, aerobic, motile, rod-shaped bacteria were isolated from the intestines of freshwater fish on two separate occasions. Colonies of both strains, JF3835(T) and JF4413, produced non-diffusible green pigment following 4-5 days incubation on Luria-Bertani agar. The most abundant fatty acids were summed feature 3 (comprising C(16 : 1)ω7c and/or C(15 : 0) iso 2-OH), C(16 : 0) and C(18 : 1)ω7c. The DNA G+C content was 62.9 mol%. Sequence analysis of the 16S rRNA gene indicated 100 % sequence similarity between the two strains. In comparison with recognized species, the new strains exhibited the greatest degree of sequence similarity with members of the Pseudomonas chlororaphis subspecies: P. chlororaphis subsp. chlororaphis (99.84 %), P. chlororaphis subsp. aurantiaca (99.75 %) and P. chlororaphis subsp. aureofaciens (99.40 %). While DNA-DNA relatedness confirmed the placement of strains JF3835(T) and JF4413 as members of the species P. chlororaphis, multilocus sequencing indicated that the strains formed a distinct cluster within it. On the basis of genotypic and phenotypic evidence, strains JF3835(T) and JF4413 represent a novel subspecies of the species P. chlororaphis, for which the name Pseudomonas chlororaphis subsp. piscium subsp. nov. is proposed. The type strain is JF3835(T) (=NCIMB 14478(T)=DSM 21509(T)).
从两种不同的淡水鱼肠道中分离到革兰氏阴性、需氧、能动、杆状细菌。两株菌（JF3835(T)和 JF4413）在 Luria-Bertani 琼脂上培养 4-5 天后，均产生非扩散性绿色色素。最丰富的脂肪酸为 sum feature 3（由 C(16 : 1)ω7c 和/或 C(15 : 0)iso 2-OH、C(16 : 0)和 C(18 : 1)ω7c 组成）、C(16 : 0)和 C(18 : 1)ω7c。DNA G+C 含量为 62.9 mol%。16S rRNA 基因序列分析表明，两株菌之间的序列相似度为 100%。与公认的物种相比，新菌株与 Pseudomonas chlororaphis 亚种的成员表现出最大的序列相似性：P. chlororaphis subsp. chlororaphis（99.84%）、P. chlororaphis subsp. aurantiaca（99.75%）和 P. chlororaphis subsp. aureofaciens（99.40%）。虽然 DNA-DNA 同源性证实了菌株 JF3835(T)和 JF4413 是 P. chlororaphis 物种的成员，但多位点测序表明，这些菌株在该物种内形成了一个独特的聚类。基于基因型和表型证据，菌株 JF3835(T)和 JF4413 代表了 P. chlororaphis 物种的一个新亚种，提议将其命名为 Pseudomonas chlororaphis subsp. piscium subsp. nov.。模式菌株为 JF3835(T)（=NCIMB 14478(T)=DSM 21509(T)）。
