Pseudomonas mangrovi sp. nov., isolated from mangrove soil.

A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated as strain TC11, was isolated from rhizosphere soil of mangrove forest (Kandeliaobovata) in Fugong village, Zhangzhou, Fujian, China. Strain TC11 grew at 15-45 °C (optimum, 35 °C), 0-8 % (w/v) NaCl (optimum, 1 %, w/v) and pH 5.5-9.5 (optimum, pH 7.5). Phylogenetic analyses revealed that strain TC11 belonged to a clade of the genus Pseudomonas and showed the highest sequence similarity of 98.4 % to Pseudomonas fluvialis ASS-1, followed by Pseudomonas oleovorans subsp.oleovorans DSM 1045 (97.9 %), Pseudomonas indoloxydans JCM 14246 (97.7 %), Pseudomonas guguanensis JCM 18416(97.6 %) and Pseudomonas alcaliphila JCM 10630 (97.5 %) on the basis of their 16S rRNA gene sequences. The DNA G+C content was 64.3 mol%. In silico DNA-DNA hybridization and average nucleotide identity values between strain TC11 and the reference strains were 19-22 % and 72-78 %, respectively. Studies based on the three housekeeping genes, rpoB, gyrB and rpoD, further confirmed that strain TC11 is a novel member of the genus Pseudomonas. The major fatty acids of strain TC11were C16 : 0, summed feature 8 (C18 : 1ω6c/C18 : 1ω7c) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The sole isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain TC11 represents a novel species of the genus Pseudomonas, for which the name Pseudomonasmangrovi sp. nov., is proposed. The type strain is TC11 (=KCTC 62159=MCCC 1K03499).